Time Course and Spacial Distribution of UV Effects on Human Skin in Organ Culture

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概要

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• Apoptosis plays an important role in eliminating cells from populations when cells have been exposed to UV irradiation and damaged. Studies of cells in culture have provided some details of the mechanisms involved when stress response genes act after exposure to UV irradiation and other environ-mental stresses. However, little is known about the responses of intact sections of human skin growing in organ culture to UV irradiation. In the work reported here, it was found that the response of organ-cultured human skin after exposure to UV irradiation is different than the response of cultured cells. At wavelengths below 300 nm, the action spectrum obtained from organ-cultured skin samples showed a lower sensitivity than that observed at 300 nm, indicating that the overlying stratum corneum and upper epidermal cell layers had probably caused a selective absorption of incident UV radiation at some wavelengths. At 3 hours after UV irradiation, p53 was phosphorylated at Ser 15 and Ser 46, and accumulated in the cell nuclei, notably after exposure to 280-320 nm wavelengths. Accumulations of Bax, active Caspase-3 and cleaved PARP were detected in apoptotic cells at 24 hours post-exposure, along with a reduction of Bcl-2 levels, notably after exposure to 300-365 nm light. This difference in apoptotic responses may result from the characteristics of the different irradiation wavelengths used, and from details in the skin's structure. The data obtained in this study using an organ-culture system utilized direct measurements of the biological effects of different wavelengths of UV lights.

• 日本放射線影響学会の論文
• 2008-05-16

著者

• TAKAHASHI Akihisa

  Department of Biology, School of Medicine, Nara Medical University

• MORI Eiichiro

  Department of Biology, School of Medicine, Nara Medical University

• OHNISHI Takeo

  Department of Biology, School of Medicine, Nara Medical University

• Ohnishi Ken

  Department Of Biology Nara Medical University

• Ohnishi Takeo

  姫路獨協大学 医療保健学部作業療法学科

• Ohnishi Takeo

  奈良県立医科大学 生物学

• Furusawa Yoshiya

  Research Center For Charged Particle Therapy National Institute Of Radiological Sciences

• OHNISHI Takeo

  Dept. Biol. Nara Med. Univ.

• Mori Eiichiro

  Department Of Biology School Of Medicine Nara Medical University

• Mori Eiichiro

  奈良県立医科大学 医学部生物学

• Mori Eiichiro

  奈良県立医科大学 医学部生物学教室

• KITAGAWA Kou

  Departments of Biology, School of Medicine, Nara Medical University

• KAKEI Saki

  Departments of Biology, School of Medicine, Nara Medical University

• TSUJINAKA Daiki

  Departments of Biology, School of Medicine, Nara Medical University

• UNNO Megumi

  Departments of Biology, School of Medicine, Nara Medical University

• NISHIKAWA Shoko

  Departments of Biology, School of Medicine, Nara Medical University

• HATOKO Mitsuo

  Departments of Dermatology, School of Medicine, Nara Medical University

• MURATA Norio

  National Institute of Basic Biology (NIBB)

• WATANABE Masakatsu

  National Institute of Basic Biology (NIBB)

• Watanabe Masami

  Particle Radiation Biology Research Reactor Institute Kyoto University

• Futami Yasuyuki

  茨城県立医療大学 放射線技術科学科

• Futami Yasuyuki

  The Institute Of Physics University Of Tsukuba

• Murata Norio

  National Institute For Basic Biology

• Ono Koji

  Particle Radiation Oncology Research Center Kyoto University

• Watanabe M

  Laboratory Of Radiation Biology Research Reactor Institute Kyoto University

• Ohnishi T

  Nara Medical Univ. Nara Jpn

• OKAICHI Kumio

  Nara Med. Univ.

• Ohnishi Takeo

  Department Of Biology Nara Medical University

• Omori Katsunori

  Institute Of Space And Astronautical Science Japan Aerospace Exploration Agency (jaxa)

• Watanabe Masani

  National Institute Of Basic Biology (nibb)

• Takahashi Akihisa

  Department Of Biology Nara Medical University

• Watanabe Masakatsu

  National Institute For Basic Biology

• Ono Koji

  京都大学原子炉実験所附属粒子線腫瘍学研究センター

• Watanabe Masami

  京都大学原子炉実験所 粒子線生物学研究分野

• Ono K

  Radiation Oncology Research Laboratory Research Reactor Institute Kyoto University

• Watanabe M

  Particle Radiation Biology Research Reactor Institute Kyoto University

• Furusawa Yoshiya

  高エネルギー加速器研究機構物質構造科学研究所

• Kakei Saki

  Departments Of Biology School Of Medicine Nara Medical University

• Ohnishi Ken

  Department Of Biology Faculty Of Science Okayama University

• Unno Megumi

  Departments Of Biology School Of Medicine Nara Medical University

• Kitagawa Kou

  Departments Of Biology School Of Medicine Nara Medical University

• Hatoko Mitsuo

  Department Of Dermatology Nara Medical University

• Hatoko Mitsuo

  Departments Of Dermatology School Of Medicine Nara Medical University

• Ohnishi Takeshi

  Dept. Biology Nara Med. Univ.

• Watanabe Masani

  Division Of Radiation Biology Faculty Of Pharmacology Of Science Nagasaki University

• Takahashi A

  Department Of Biology Nara Medical University

• Takahashi Akihisa

  奈良県立医科大学 生物学

• Watanabe Masami

  Lab. Of Radiation Biology Div. Of Radiation Life Sci. Res. Reactor Inst. Kyoto Univ.

• Ohnishi Ken

  Dept. Biology Nara Med. Univ.

• Ohnishi Takeo

  Nara Medical University

• Tsujinaka Daiki

  Departments Of Biology School Of Medicine Nara Medical University

• Nishikawa Shoko

  Departments Of Biology School Of Medicine Nara Medical University

• Furusawa Yoshiya

  Div. Of Accelerator Res. Natl. Inst. Of Radiol. Sci.

• Ogura Koichi

  College Of Industrial Technology Nihon University

• Ohnishi Takeo

  Dept. Of Biol. Nara Medical Univ.

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