Analysis of Apoptosis-related Gene Expression after X-ray Irradiation in Human Tongue Squamous Cell Carcinoma Cells Harboring Wild-type or Mutated p53 Gene

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概要

• 論文の詳細を見る

• Mutations in the p53 tumor suppressor gene have recently been reported to have an impact on clinical trials of several human tumors, including head and neck cancers. To confirm the p53-dependence of X-ray induced apoptosis, we used two cell lines derived from a human squamous cell carcinoma (SAS) with identical genetic backgrounds, except for the p53 gene, which are SAS/mp53 cells with mp53 and SAS/neo cells with wtp53. We previously reported that the radiosensitivity, Caspase-3 activity and apoptosis frequency in SAS/neo cells were clearly high as compared with SAS/mp53 cells. In order to elucidate the expression of apoptosis-related genes after irradiation, we used cDNA array analysis. The expressions of apoptosis-inductive genes, such as DFF40, Caspase-3, Caspase-8, Caspase-9, Caspase-10 and CRADD, were increased by X-ray irradiation in SAS cells with wtp53, but not in SAS cells expressing mp53. These results suggest that the X-ray sensitivity of wtp53 cells may come from the expression of these apoptosis-related genes.

• 日本放射線影響学会の論文

著者

• TAKAHASHI Akihisa

  Department of Biology, School of Medicine, Nara Medical University

• OHNISHI Takeo

  Department of Biology, School of Medicine, Nara Medical University

• 山口 浩一

  Department Of Electronic Engineering

• Ohnishi Ken

  Department Of Biology Nara Medical University

• KIRITA Tadaaki

  Department of Oral and Maxillofacial Surgery, School of Medicine, Nara Medical University

• IMAI Yuichiro

  Department of Oral and Maxillofacial Surgery, Nara Medical University

• Ono Koji

  Particle Radiation Oncology Research Center Kyoto University

• OKAICHI Kumio

  Nara Med. Univ.

• Kirita T

  Department Of Oral And Maxillofacial Surgery School Of Medicine Nara Medical University

• Ohnishi Takeo

  Department Of Biology Nara Medical University

• Omori Katsunori

  Institute Of Space And Astronautical Science Japan Aerospace Exploration Agency (jaxa)

• 山口 浩一

  電気通信大学電子工学科

• YAMAGUCHI Katsumi

  Japan Space Utilization Promotion Center

• Takahashi Akihisa

  Department Of Biology Nara Medical University

• Yamaguchi Kiyoshi

  Faculty Of Engineering Osaka Institute Of Technology

• Yamaguchi Katsumi

  Department Of Mechanical Engineering Nagoya University

• Ono K

  Radiation Oncology Research Laboratory Research Reactor Institute Kyoto University

• Yuki Kazue

  Otorhinolaryngology Nara Medical University

• Ohnishi Ken

  Department Of Biology Faculty Of Science Okayama University

• Yasumoto Junichi

  Departments Of Oral And Maxillofacial Surgery Nara Medical University

• Ohnishi Takeshi

  Dept. Biology Nara Med. Univ.

• Takahashi A

  Department Of Biology Nara Medical University

• Kirita Tadaaki

  Department Of Oral And Maxillofacial Surgery Nara Medical University

• Imai Yuichiro

  Department Of Oral And Maxillofacial Surgery Nara Medical University

• 山口 浩一

  電気通信大学電気通信学部電子工学科

• YAMAGUCHI Kazufumi

  Wireless Research Laboratory, Matsushita Electric Industrial Co., Ltd.

• Ohnishi Takeo

  Nara Medical University

• Yamaguchi Kazuhiko

  Institute For Laser Science University Of Electro-communications

• Ogura Koichi

  College Of Industrial Technology Nihon University

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