Changes in the Translatable RNA Population during Abscisic Acid Induced Freezing Tolerance in Bromegrass Suspension Culture
スポンサーリンク
概要
- 論文の詳細を見る
Abscisic acid (ABA) has been shown to increase freezing tolerance of bromegrass (Bromus inermis Leyss cv. Manchar) cell suspension cultures from a LT_<50> (the temperature at which 50% cells were killed) of -7 to -30℃ in 5 days at 23℃. Our objective was to study the qualitative changes in the translatable RNA population during ABA induced frost tolernace. In vitro translation products of poly(A)+ RNA isolated from bromegrass cells with or without 75 μM ABA treatment for various periods of time were separated by 2D-PAGE and visualized by fluorography. SDS soluble proteins from the same treatments were also separated by 2D-PAGE. After 5 days treatment, at least 22 new or increased abundance SDS soluble polypeptides were observed. From fluorographs, 29 novel or increased abundance in vitro translation products could be detected. The pattern of changes between ABA induced SDS-soluble proteins and translation products from the 2D gels were similar. A time course study (0-7 days) showed that 17 of the 29 translation products were detected after 1 day ABA treatment, and at least 14 were present after 1 h. Cold treatment (+4℃) induced fewer changes in the pool of translatable RNA than with ABA treatment. Three translation products induced by cold appear to be similar to 3 of the ABA induced translation products. The majority of the ABA inducible translatable RNAs appeared at 10μM Or higher which coincides with the induction of freezing tolerance. Many of these ABA inducible RNAs persisted 7 days after ABA was removed from the media and correspondingly the LT_<50> (-17℃) was still well above the control level (-7℃). The results suggest that ABA alters the pool of translatable RNAs during induction of freezing tolerance in bromegrass suspension culture cells.
- 日本植物生理学会の論文
著者
-
Lee Stephen
Department Of Horticulture Oregon State University
-
Lee Stephen
Department Of Electronic Engineering City University Of Hong Kong
-
Chen Tony
Department Of Horticulture 4017 Ag And Life Sciences
-
Chen Tony
Department Of Horticulture Oregon State University
-
Fuchigami Leslie
Department Of Horticulture Oregon State University
関連論文
- 1. Genetic Engineering of Cold-Tolerant Tomato via Glycinebetaine Biosynthesis
- ROLE OF GLYCINEBETAINE IN FREEZING TOLERANCE : A TRANSGENIC APPROACH
- A CHLAMYDOMONAS MUTANT DEFECTIVE IN THE CONVERSION OF VIOLAXANTHIN TO NEOXANTHIN IN ABA BIOSYNTHESIS
- Enhanced Germination under High-Salt Conditions of Seeds of Transgenic Arabidopsis with a Bacterial Gene(codA)for Choline Oxidase
- ISOLATION OF CHLAMYDOMONAS MUTANT IMPAIRED AT THE INTERVENING STEP IN ABSCISIC ACID BIOSYNTHESIS
- Use of Chaotic Switching in Electronic Ballasts(Nonlinear Phenomena)(Nonlinear Theory and its Applications)
- High Performance Liquid Chromatography Analysis of Reduced and Oxidized Glutathione in Woody Plant Tissues
- Sertoliform cystadenoma of the rete testis
- Changes in Protein Profiles of Poplar Tissues during the Induction of Bud Dormancy by Short-Day Photoperiods
- Exogenous Application of Glycinebetaine Increases Chilling Tolerance in Tomato Plants
- STUDIES ON A GENE INVOLVED IN THE ISOMERIZATION FROM VIOLAXANTHIN TO NEOXANTHIN IN Chlamydomonas reinhardtii
- Changes in the Translatable RNA Population during Abscisic Acid Induced Freezing Tolerance in Bromegrass Suspension Culture
- Glutathione Content in Peach Buds in Relation to Development and Release of Rest : ENVIRONMENTAL AND STRESS RESPONSES
- Metabolic Alterations Associated With Abscisic Acid-Induced Frost Hardiness in Bromegrass Suspension Culture Cells
- Cloning and Functional Characterization of a Gene for Capsanthin-Capsorubin Synthase from Tiger Lily (Lilium lancifolium Thunb. 'Splendens')