Purification of the Cytosolic CuZn-Superoxide Dismutase (CuZn-SOD) of Marchantia paleacea var. diptera and its Resemblance to CuZn-SOD from Chloroplasts

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概要

• 論文の詳細を見る

• Suspension-cultured cells of Marchantia paleacea var. diptera contain a single form of CuZn-superoxide dismutase (SOD; EC 1.15.1.1) which is localized in the cytosol. SOD activity was found in cells cultured under heterotrophic, photoheterotrophic and photoautotrophic conditions. The CuZn-SOD was purified to homogeneity from liverwort cells that had been cultured heterotrophically. Its molecular mass was 32.6 kDa, and it contained 17.5 kDa subunits, an indication that the enzyme is a homodimer. The enzyme had peaks of absorption at 252, 258 and 264 nm in the ultraviolet region, due to the presence of phenyl-alanine, and a peak at 680 nm in the visible region, which is characteristic of CuZn-SODs from chloroplasts. The amino acid sequence of the amino-terminal region of the enzyme exhibited a very high degree of homology to those of chloroplast CuZn-SODs. An antiserum raised against the CuZn-SOD from liverwort cross-reacted more strongly with the enzyme from spinach chloroplasts, than with the enzyme from spinach cytosol. These results indicate that the CuZn-SOD of liverwort resembles CuZn-SOD in chlo-roplasts even though the former is located in the cytosol.

• 日本植物生理学会の論文

著者

• TAKIO Susumu

  Department of Biological Science, Faculty of Science, Hiroshima University

• Takio S

  Department Of Biological Science Faculty Of Science Kumamoto University

• Takio Susumu

  Center For Marine Environment Studies Kumamoto Univ.

• Takio Susumu

  Center For Marine Environment Studies Kumamoto University

• Yamamoto Isamu

  Deparment Of Biological Science Graduate School Of Science Hiroshima University

• Tanaka Katsuyuki

  Dept. Biol. Sci. Fac. Sci. Hiroshima Univ.

• Tanaka Katsuyuki

  Department Of Mechanical System Engineering School Of Engineering University Of Shiga Prefecture

• Satoh Tadashi

  Department Of Biological Chemistry College Of Bioresource Sciences Nihon University

• Satoh Toshio

  Department Of Bio-organic Medicinal Chemistry Faculty Of Pharmaceutical Sciences Tokushima Bunri Uni

• Tanaka K

  Dept. Biol. Sci. Fac. Sci. Hiroshima Univ.

• Yamamoto I

  Deparment Of Biological Science Graduate School Of Science Hiroshima University

• Tanaka Katsuyuki

  Department Of Applied Physics Faculty Of Science Tokyo Institute Of Technology

• Shiono Tadahiko

  Department Of Biological Science Graduate School Of Science Hiroshima University

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