Efficient plant regeneration and micropropagation from callus derived from mature zygotic embryo of Larix gmelinii

概要

論文の詳細を見る
Calli derived from mature zygotic embryos and seedling explants of Larix gmelinii were used to investigate for organogenesis. After 4 weeks of culture on half-strength DCR medium containing BA, numerous adventitious buds formed on the callus tissues which were derived from zygotic embryos, but none formed on calli generated from seedling explants. Rates of callus proliferation and adventitious bud formation were higher when the callus was transferred to medium supplemented with activated charcoal. To induce shoot elongation, multiple shoots were separated and transferred to medium with 0.05% activated charcoal. To induce adventitious root formation, elongated shoots of 1.5-2.0 cm in length were cultured in half-strength MS medium containing 0.3 mg l-1 NAA and 0.3 mg l-1 IBA. To assess genetic homogeneity and somatic variation, chromosomes of regenerated plantlets were observed. No numerical or structural changes to the chromosomes were found in regenerated plantlets using this system.
日本植物細胞分子生物学会の論文
2004-06-01