Cryptic Polyadenylation of Transcripts of an RNA Virus Gene Introduced into Tobacco Plants
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概要
- 論文の詳細を見る
We constructed an expression vector for the coat protein (CP) gene and the 3′ untranslated region (3′ UTR) of RNA virus (sweet potato feathery mottle virus severe strain (SPFMV-S)) lacking a foreign terminator. Out of seven transgenic tobacco plants, expression of the transgene was observed in six plants. RT-PCR analysis revealed that the transcripts had a poly(A) tail, and in most of them, polyadenylation occurred on the 5′ side of the 3′ UTR. These results suggest that the viral sequence contains a cryptic polyadenylation signal that permits 3′-end processing of the transcripts.
- 社団法人 日本農芸化学会の論文
- 2005-10-23
著者
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Takio Susumu
Center For Marine Environment Studies Kumamoto Univ.
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Takio Susumu
Center For Marine Environment Studies Kumamoto University
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SAITO Akira
National Institute of Agrobiological Resources
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KIMURA Takashi
National Research Institute for Metals
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Takio Susumu
Center For Marine Environment Studies
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Hanada Kaoru
National Agricultural Research Center For Kyushu Okinawa Region:(present Address) National Institute
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Hanada Kaoru
National Institute Of Agrobiological Sciences
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Hanada Kaoru
National Agricultural Research Center For Kyushu Okinawa Region
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TANAKA Yuko
National Agricultural Research Center for Kyushu Okinawa Region
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Kimura Takashi
National Agricultural Res. Center For Kyushu Okinawa Region National Agriculture And Food Res. Organ
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Tanaka Yuko
National Agricultural Research Center For Kyushu Okinawa Region National Agriculture And Bio-oriente
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Kimura Takashi
National Agricultural Research Center For Kyushu Okinawa Region National Agriculture And Bio-oriente
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Saito Akira
National Agricultural Research Center For Kyushu Okinawa Region
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