Involvement in Denitrification of the napKEFD ABC Genes Encoding the Periplasmic Nitrate Reductase System in the Denitrifying Phototrophic Bacterium Rhodobacter sphaeroides f. sp. denitrificans
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概要
- 論文の詳細を見る
Seven genes, napKEFDABC, encoding the periplasmic nitrate reductase system were cloned from the denitrifying phototrophic bacterium Rhodobacter sphaeroides f. sp. denitrificans IL106. Two transmembrane proteins, NapK and NapE, an iron-sulfur protein NapF, a soluble protein NapD, a catalytic subunit of nitrate reductase precursor NapA, a soluble c-type diheme cytochrome precursor NapB, and a membrane-anchored c-type tetraheme cytochrome NapC were deduced as the gene products. Every mutant in which each nap gene was disrupted by Ω-cassette insertion lost nitrate reductase activity as well as the ability of cells to grow with nitrate under anaerobic-dark conditions. A transconjugant of the napD-disrupted mutant with a plasmid bearing the napKEFDABC genes recovered both nitrate reductase activity and nitrate-dependent anaerobic-dark growth of cells. Denitrification activity, which was not observed in the napD mutant, was also restored by the conjugation. These results indicate that the periplasmic nitrate reductase encoded by the napKEFDABC genes is the enzyme responsible for denitrification in this phototroph, although the presence of a membranebound nitrate reductase has been reported in the same strain.
- 社団法人日本農芸化学会の論文
- 1999-03-23
著者
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Satoh Toshio
Department of Biological Science, Faculty of Science, Hiroshima University
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Yamamoto Isamu
Department of Biological Sciences, Faculty of Science, Hiroshima University
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TAKIO Susumu
Department of Biological Science, Faculty of Science, Hiroshima University
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Takio S
Department Of Biological Science Faculty Of Science Kumamoto University
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Takio Susumu
Center For Marine Environment Studies Kumamoto Univ.
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Takio Susumu
Center For Marine Environment Studies Kumamoto University
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Yamamoto Isamu
Deparment Of Biological Science Graduate School Of Science Hiroshima University
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Satoh Tadashi
Department Of Biological Chemistry College Of Bioresource Sciences Nihon University
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Liu Hui-ping
Dept. Biol. Sci. Fac. Sci. Hiroshima Univ.
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Satoh Toshio
Department Of Bio-organic Medicinal Chemistry Faculty Of Pharmaceutical Sciences Tokushima Bunri Uni
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Yamamoto I
Deparment Of Biological Science Graduate School Of Science Hiroshima University
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Satoh T
Bio-organic Chemistry Laboratory Graduate School Of Bioresource Sciences Nihon University
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LIU Hui-Ping
Department of Biological Science, Faculty of Science, Hiroshima University
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Shiono Tadahiko
Department Of Biological Science Graduate School Of Science Hiroshima University
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