Appearance of Nitrite Reducing Activity of Cytochrome c upon Heat Denaturation(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
The appearance of NO_2^- reducing activity of cytochrome c (Cyt c) upon heat denaturation was investigated with equine heart Cyt c. Denatured equine heart Cyt c (dCyt c), which was treated at 100℃ for 30 min, had NO_2^- reducing activity in the presence of dithionite and methylviologen in an aqueous solution under anaerobic conditions. In contrast, hemoglobin and myoglobin had no such activity under the same conditions. Using spectroscopic methods, we found that the appearance of this activity in the Cyt c was due to the following intramolecular changes : unfolding of the peptide chain, exposure of the heme, dissociation of the sixth ligand methionine sulfur, and appearance of autoxidizability. The dCyt c catalyzed NO_2^- reduction to NH_4^+ via ferrous-NO complexes, and this reaction was a 6-electron and 8-proton reduction. Sepharose-immobilized dCyt c had activity similar strength to that in solution. The resin retained the activity after five uses and even after storage for 1 year. On the basis of these results, we concluded that Cyt c acquired a new catalytic activity upon heat treatment, unlike to other familiar biological molecules.
- 社団法人日本農芸化学会の論文
- 2002-10-23
著者
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NISHIO Toshiyuki
Department of Pediatrics, Tohoku University Graduate School of Medicine
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Ogawa Masahiro
Department of Cardiology, Fukuoka University School of Medicine
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Satoh Tadashi
Department of Public Health, Niigata University School of Medicine
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KAWACHI Ryu
Department of Applied Biological Chemistry, The University of Tokyo
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YAMADA Shigehiro
ORYNOVA K. K.
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Nishio Toshiyuki
Biomolecular Chemistry Lab. Graduate School Of Bioresource Sciences Nihon Univ.
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Nishio Toshiyuki
Department Of Biological Chemistry College Of Bioresource Sciences Nihon University
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Satoh Tadashi
Department Of Biological Chemistry College Of Bioresource Sciences Nihon University
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Ogawa M
Laboratory Of Bio-organic Chemistry College Of Bioresource Sciences Nihon University
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Ogawa Masahiro
Department Of Applied Biological Science Faculty Of Agriculture Kagawa University
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Kawachi Ryu
Department Of Applied Biological Chemistry The University Of Tokyo
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Kawachi Ryu
Laboratory Of Bio-organic Chemistry College Of Bioresource Sciences Nihon University
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Yamada S
Department Of Biological Chemistry College Of Bioresource Sciences Nihon University:(present Address
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Oku Tadatake
Biomolecular Chemistry Lab. Graduate School Of Bioresource Sciences Nihon Univ.
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Oku Tadatake
Department Of Agricultural Chemistry College Of Agriculture And Veterinary Medicine Nihon University
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YAMADA Seiji
Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
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SURUGA Kohei
Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
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HAMA Toshiyuki
Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
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Suruga Kohei
Bio-organic Chemistry Laboratory Graduate School Of Bioresource Sciences Nihon University
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Hama Toshiyuki
Bio-organic Chemistry Lab. Graduate School Of Bioresource Sciences Nihon Univ.
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Satoh T
Bio-organic Chemistry Laboratory Graduate School Of Bioresource Sciences Nihon University
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Yamada S
Department Of Biology And Geosciences Faculty Of Science Shizuoka University
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Ogawa Masahiro
Department Of Anesthesiology Nihon University School Of Medicine
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Shiono Tadahiko
Department Of Biological Science Graduate School Of Science Hiroshima University
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Yamada Seiji
Department of Applied Chemistry, Faculty of Engineering, Kansai University
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