Family M42 aminopeptidase from the syntrophic bacterium Symbiobacterium thermophilum : Characterization using recombinant protein(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

• 論文の詳細を見る

• The chromosomal DNA of the syntrophic thermophile Symbiobacterium thermophilum contains open reading frames of the genes encoding family M42 aminopeptidases, Pep1079, Pep1080, and Pep1081. To characterize these peptidases, the genes were cloned into Escherichia coli and overexpressed. Our experiments using the recombinant proteins confirmed that Pep1079, Pep1080, and Pep1081 are components of arginyl or lysinyl aminopeptidases that require Co^<2+> for enzymatic activity. Coexistence of Pep1079 and Pep1080 is necessary for expressing high peptidase activity. Pep1081 enhances the activity of Pep1079 and Pep1080.

• 社団法人日本生物工学会の論文

著者

• NISHIO Toshiyuki

  Department of Pediatrics, Tohoku University Graduate School of Medicine

• Ogawa Masahiro

  Department of Cardiology, Fukuoka University School of Medicine

• Hakamata Wataru

  Biomolecular Chemistry Lab. Graduate School Of Bioresource Sciences Nihon Univ.

• Nishio Toshiyuki

  Biomolecular Chemistry Lab. Graduate School Of Bioresource Sciences Nihon Univ.

• Nishio Toshiyuki

  Department Of Biological Chemistry College Of Bioresource Sciences Nihon University

• Ogawa M

  Laboratory Of Bio-organic Chemistry College Of Bioresource Sciences Nihon University

• Ogawa Masahiro

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University

• Oku Tadatake

  Biomolecular Chemistry Lab. Graduate School Of Bioresource Sciences Nihon Univ.

• Oku Tadatake

  Department Of Agricultural Chemistry College Of Agriculture And Veterinary Medicine Nihon University

• Hirano Takako

  Biomolecular Chemistry Lab. Graduate School Of Bioresource Sciences Nihon Univ.

• Hirano Takako

  Life Science Research Center College Of Bioresource Sciences Nihon University

• Yoshikawa Keiko

  Department Of Chemistry And Life Science College Of Bioresource Sciences Nihon University

• HAKAMATA Wataru

  Department of Biological Chemistry, College of Bioresource Sciences, Nihon University

• Kumaki Yasuko

  Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University

• Iwasawa Naoya

  Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University

• Yagi Takurou

  Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University

• Iwasawa Naoya

  Department Of Chemistry And Life Science College Of Bioresource Sciences Nihon University

• Yagi Takurou

  Department Of Chemistry And Life Science College Of Bioresource Sciences Nihon University

• Kumaki Yasuko

  Department Of Chemistry And Life Science College Of Bioresource Sciences Nihon University

• Ogawa Masahiro

  Department Of Anesthesiology Nihon University School Of Medicine

• Yoshikawa Keiko

  Department of Applied Chemistry, Institute of Colloid and Interface Science, Science University of Tokyo

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