Characterization and Amino Acid Sequences of Cytochromes c_6 from Two Strains of the Green Alga Chlorella vulgaris

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概要

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• Cytochromes c_6 from the green algae Chlorella vulgaris CK-5 (CK5cyc6) and C. vulgaris CK-22 (CK22cyc6) were characterized and their amino acid sequences were analyzed. CK5cyc6 had a molecular mass of 9.3 kDa, isoelectric points of 3.0 (reduced) and 3.6 (oxidized), and a redox potential of +362 mV at pH 7.0. CK22cyc6 had a molecular mass of 9.5 kDa, isoelectric points of 2.9 (reduced) and 3.5 (oxidized), and a redox potential of +355 mV at pH 7.0. The absorption spectra of both cytochromes c_6 showed 4 maxima in reduced form, and 2 maxima and a weak peak at 695 nm in oxidized form. The pyridine ferrohemochrome spectra indicated that their prosthetic group was heme c. These physicochemical properties were similar to those of other algal cytochromes c_6. The amino acids (88 residues) of CK5cyc6 and CK22cyc6 were sequenced and the sequence motif-CXXCH-, which is typical of the heme-binding site of c-type cytochrome, was clearly confirmed in both cytochromes. Twenty-six amino acid residues were substituted, and the similarity score of each of them was 70.45%.

• 社団法人日本農芸化学会の論文
• 2000-03-23

著者

• NISHIO Toshiyuki

  Department of Pediatrics, Tohoku University Graduate School of Medicine

• Isogai Yasuhiro

  The Institute Of Physical And Chemical Research (riken)

• YAMADA Shigehiro

  ORYNOVA K. K.

• Nishio Toshiyuki

  Biomolecular Chemistry Lab. Graduate School Of Bioresource Sciences Nihon Univ.

• Nishio Toshiyuki

  Department Of Biological Chemistry College Of Bioresource Sciences Nihon University

• Tanaka Y

  Hiroshima Univ. Higashi‐hiroshima Jpn

• Yamada S

  Department Of Biological Chemistry College Of Bioresource Sciences Nihon University:(present Address

• Oku Tadatake

  Biomolecular Chemistry Lab. Graduate School Of Bioresource Sciences Nihon Univ.

• Oku Tadatake

  Department Of Agricultural Chemistry College Of Agriculture And Veterinary Medicine Nihon University

• YAMADA Seiji

  Department of Biological Chemistry, College of Bioresource Sciences, Nihon University

• NAKAMURA Toshio

  Chlorella Industry, Co., Ltd.

• TANAKA Yoshimasa

  Chlorella Industry, Co., Ltd.

• Yamada S

  Department Of Biology And Geosciences Faculty Of Science Shizuoka University

• Tanaka Yoshimasa

  Chlorella Industry Co. Ltd.

• Nakamura Toshio

  Chlorella Industry Co. Ltd.

• Yamada Seiji

  Department of Applied Chemistry, Faculty of Engineering, Kansai University

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