Production and Secretion of a Recombinant Vibrio parahaemolyticus Chitinase by Escherichia coli and Its Purification from the Culture Medium

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概要

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• An open reading frame encoding the chitinase gene and its signal sequence was cloned from the Vibrio parahaemolyticus KN1699 genome. An expression plasmid containing the gene was introduced into Escherichia coli cells, and recombinant chitinase (Pa-rChi) was produced and secreted into the culture medium with the aid of the signal peptide. Pa-rChi was purified and its substrate specificity was determined.

• 社団法人 日本農芸化学会の論文

著者

• NISHIO Toshiyuki

  Department of Pediatrics, Tohoku University Graduate School of Medicine

• Oku Tadatake

  Department Of Agricultural Chemistry College Of Agriculture And Veterinary Medicine Nihon University

• Suruga Kohei

  Bio-organic Chemistry Laboratory Graduate School Of Bioresource Sciences Nihon University

• SAITO Kaori

  Department of Applied Chemistry, Faculty of Engineering, Osaka University

• KADOKURA Kazunari

  Department of Biological Chemistry, College of Bioresource Sciences, Nihon University

• SAKAMOTO Yusuke

  Department of Biological Chemistry, College of Bioresource Sciences, Nihon University

• IKEGAMI Takanori

  Department of Biological Chemistry, College of Bioresource Sciences, Nihon University

• HIRANO Takako

  Department of Biological Chemistry, College of Bioresource Sciences, Nihon University

• HAKAMATA Wataru

  Department of Biological Chemistry, College of Bioresource Sciences, Nihon University

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