Mechanisms of Nitroso Compound-Induced Inhibition of Superoxide Generation in Neutrophils: Fluorescence Quenching of Perylene by Nitroso-Compounds in the Membrane Fractions of Neutrophils.
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概要
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To investigate the mechanism of nitroso compound-induced inhibition of the respiratory burst in neutrophils, we studied fluorescence quenching of perylene by nitroso-compounds in the membrane fractions of neutrophils at 17, 27, and 37°C and the reagent-induced inhibition of superoxide generation at 28 and 37°C. With increasing temperature, the quenching of perylene fluorescence and inhibition of superoxide generation by nitrosobenzene (NB) were both diminished, while those by 2-nitrosotoluene (NT) were both enhanced. The temperature dependence of the inhibition constants and the quenching constants indicates that the binding of NB is exothermic (ΔH=-27 kJ/mol for inhibition and ΔH=-29 kJ/mol for quenching) and essentially enthalpy-driven. On the other hand, that of NT is endothermic (ΔH=+16 kJ/mol for inhibition and quenching) and essentially entropydriven. Quenching studies of perylene fluorescence in synthetic vesicles made of endogenous polar lipids of neutrophils showed that the enthalpy changes of NB- and NT-binding with perylene in lipids were similar to each other. Moreover, their values were in good agreement with that of NT, but not of NB, in the membrane fractions, an assembly of proteins and lipids, of neutrophils. These results suggest that NB inhibits the activity by binding to proteins in the membrane, whereas inhibition by NT occurs through hydrophobic interaction with lipids and/or proteins.
- 社団法人 日本生化学会の論文
著者
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Nakata Munehiro
The Institute Of Physical And Chemical Research (riken)
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Isogai Yasuhiro
The Institute Of Physical And Chemical Research (riken)
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NASUDA-KOUYAMA Akemi
The Institute of Physical and Chemical Research (RIKEN)
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IIZUKA Tetsutaro
The Institute of Physical and Chemical Research (RIKEN)
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