Evaluation of Induction of CYP3A mRNA Using the HepG2 Cell Line and Reverse Transcription-PCR
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概要
- 論文の詳細を見る
Cytochrome P-450 3A (CYP3A) is a drug-metabolizing enzyme dominant in the human liver. We have designed a useful method for evaluation of induction of CYP3A mRNA by various drugs using HepG2 cells known to retain liver-cellular functions. Using semi-quantitative reverse transcription-PCR (RT-PCR), we demonstrated that cultured HepG2 cells constitutively expressed CYP3A mRNA. This mRNA was expressed at high levels in culture for several days and was further induced by several drugs (e.g. rifampicin (RFP), dexamethasone). Treatment of HepG2 cells with RFP induced CYP3A mRNA in a dose- and time-dependent manner. Cells in culture for 48 h with 1 and 50 μmol/l RFP increased 2.7- and 5.0-fold in CYP3A mRNA expression in comparison with untreated controls, respectively. In contrast, no change in the amount of CYP3A mRNA was observed when the cells were treated with cimetidine which has been shown to inhibit CYP3A activity. Our method using a combination of HepG2 cells and RT-PCR allowed evaluation of the degree of induction of CYP3A mRNA both easily and rapidly.
- 公益社団法人日本薬学会の論文
- 1999-01-15
著者
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Azuma Junichi
Department of Clinical Evaluation of Medicines and Therapeutics, Graduate School of Pharmaceutical S
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Yamamoto Isamu
Department of Biological Sciences, Faculty of Science, Hiroshima University
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Zhou Q
Chinese Univ. Hong Kong Hkg
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Zhou Qian
大阪大学 薬研究 臨薬効評価
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Zhou Qian
Department Of Clinical Evaluation Of Medicines And Therapeutics Graduate School Of Pharmaceutical Sc
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Zhou Qian
Department Of Chemistry Wuhan University
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Azuma Junichi
Department Of Clinical Evaluation Of Medicines And Therapeutics Graduate School Of Pharmaceutical Sc
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Yamamoto Isamu
Deparment Of Biological Science Graduate School Of Science Hiroshima University
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Yamamoto I
Department Of Clinical Evaluation Of Medicines And Therapeutics Graduate School Of Pharmaceutical Sc
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SUMIDA Akihiko
Department of Clinical Evaluation of Medicines and Therapeutics, Graduate School of Pharmaceutical S
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MORISAKI Tomoko
Department of Clinical Evaluation of Medicines and Therapeutics, Graduate School of Pharmaceutical S
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Sumida Akihiko
Department Of Clinical Evaluation Of Medicines And Therapeutics Graduate School Of Pharmaceutical Sc
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Morisaki T
Department Of Clinical Evaluation Of Medicines And Therapeutics Graduate School Of Pharmaceutical Sc
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