Isolation and characterization of nitrate reductase-deficient cell lines from a haploid culture of peony.
スポンサーリンク
概要
- 論文の詳細を見る
Six chlorate-resistant (CR) cell lines were established from survivors after plating mutagenized and non-mutagenized cells of Kp20, a Paeonia haploid cell line, onto solid medium containing 30mM KClO3 and amino acids as the sole nitrogen source (AA-chlorate medium). Five millimolar N-ethyl-N-nitrosourea (ENU) was used as the mutagen. None of the 6 CR lines grew on medium containing nitrate as the sole nitrogen source (NO3-medium). Of these CR lines, 5 completely lacked in vivo nitrate reductase (NR) activity and one showed low (about 11% of that of the wild type) NR activity. In all CR lines except for one, the percentage occupied by haploid cells increased to 83-95% compared with 73% in the wild type. This suggested that mutation may have occurred to advantage in the haploid cells.
- 日本遺伝学会の論文
著者
-
Ono Kanji
Department Of Biological Science Faculty Of Science Kumamoto University
-
TAKAHARA Tohru
Department of Biology, Faculty of Science, Kumamoto University
関連論文
- Chromosomal Aberrations of Cultured Cells and Regenerated Plantlets in the Diploid Scilla scilloides (2n = 16, AA)
- Effects of Antibiotics that Inhibit the Bacterial Peptidoglycan Synthesis Pathway on Moss Chloroplast Division
- Does Potassium Ion Concentration Affect Lung Preservation?
- On the Chromosome Number in Mniaceae
- Fracture-Mode Classification in Glass-Fiber Composites by Acoustic Emission Source Wave Characterization and Autoregressive Coefficients
- Inhibition of Plastid Division by Ampicillin in the Pteridophyte Selaginella nipponica Fr. et Sav.
- Modal Analysis of Hollw Cylindrical Guided Waves and Applications
- Biosystematic Studies on the Genus Isoetes (Isoetaceae) in Japan. III. Variability within Qualitative and Quantitative Morphology of Spores
- Biosystematic Studies on the Genus Isoetes in Japan I. Variations of the Somatic Chromosome Numbers
- Ampicillin Inhibits Chloroplast Division in Cultured Cells of the Liverwort Marchantia polymorpha
- Efficient plant regeneration and micropropagation from callus derived from mature zygotic embryo of Larix gmelinii
- Photosynthetic Electron Transport Differentially Regulates the Expression of Superoxide Dismutase Genes in Liverwort, Marchantia paleacea var. diptera
- Photosynthetic Electron Transport Regulates the Stability of the Transcript for the Protochlorophyllide Oxidoreductase Gene in the Liverwort, Marchantia paleacea var. diptera
- Formation of Hydrogen Peroxide by NAD(P)H Oxidation with Isolated Cell Wall-Associated Peroxidase from Cultured Liverwort Cells, Marchantia polymorpha L.
- Growth Characteristics of Liverwort Cells, Marchantia paleacea var. diptera, in a Photoautotrophic Suspension Culture
- Enzyme Electrophoretic Evidence for Diploidy in Japanese Woodwardia japonica (L. f.) Sm. (Blechnaceae, Pteridophyte) with 2n=68
- Effect of Fracture Dynamics in Thin Plate on the Waveform and Radiation Pattern of the Lamb Waves
- Induction of Haploid Callus from Isolated Microspores of Peony in vitro
- Chloroplast Division in Cultured Cells of the Hornwort Anthoceros punctatus
- Karyomorphological Studies on Woodwardia sensu lato of Japan
- Changes in Plastid DNA Content during the Life Cycle of the Hornwort Anthoceros punctatus L.
- Somatic Embryogenesis Pathways in Cultured Allium fistulosum Cells
- Chromosomal Variation in Callus Lines and Regenerated Plantlets from Three Cultivars of Allium fistulosum L. (2n=16)
- HAPLOID CALLUS FORMATION FROM ANTHER CULTURES IN A CULTIVAR OF PAEONIA
- Isolation and characterization of nitrate reductase-deficient cell lines from a haploid culture of peony.
- Growth characteristics and chromosomal behavior of cell suspension lines established from pollen callus of peony.
- Modal Analysis of Hollow Cylindrical Guided Waves and Applications