Construction of physical map and mapping of chromosomal virulence genes of the biovar 3 Agrobacterium (Rhizobium vitis) strain K-Ag-1
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概要
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Most plant pathogenic Agrobacterium strains have been classified into three biovars, “biovar 1 (A. tumefaciens; Rhizobium radiobacter), biovar 2 (A. rhizogenes; R. rhizogenes) and biovar 3 (A. vitis; R. vitis)”. The bacteria possess diverse types of genomic organization depending on the biovar. Previous genomic physical maps indicated difference in location of rDNA and chromosomally-coded virulence genes between biovar 1 and 2 genomes. In order to understand biovar 3 genome and its evolution in relation to the biovar 1, 2 and 3 genomes, we constructed physical map of a pathogenic biovar 3 strain K-Ag-1 in this study. Its genome consisted of two circular chromosomes (3.6 and 1.1 Mbp in length), and three plasmids (560, 230 and 70 kbp). Gene mapping based on the physical map showed presence of two rDNA loci in the larger chromosome and at least one rDNA locus in the smaller chromosome. Six chromosomal virulence genes, namely chvA, chvD, chvE, glgP, exoC and ros were found in the larger chromosome and not in the smaller chromosome. The location of rDNA loci is similar with that of biovar 1 genome, whereas the location of chromosomal virulence genes is similar with that of biovar 2 genome despite of the closer 16S-rRNA based phylogenetic relation of biovar 3 with biovar 1 than with biovar 2. Genomic PFGE RFLP analysis revealed that the K-Ag-1 strain, which was isolated on a kiwifruit plant in Japan, has the closest intra-species relation with two strains isolated from grapevine plants in Japan among eight biovar 3 strains examined. This datum suggests that the line of the strain is a major one in biovar 3 in Japan. Evolution of the genome of the strain is discussed based on the data.
- 2006-12-25
著者
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SAWADA Hiroyuki
National Institute for Agro-Environmental Sciences
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Tanaka Katsuyuki
Department Of Mechanical System Engineering School Of Engineering University Of Shiga Prefecture
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SUZUKI Katsunori
Department of Biological Science, Graduate School of Science, Hiroshima University
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Matsui Hiroki
Department Of Mechanical Engineering Kyoto University
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Sawada Hiroyuki
National Institute For Agrobiological Sciences
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URBANCZYK Henryk
Department of Biological Science, Graduate School of Science, Hiroshima University
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Urbanczyk Henryk
Department Of Biological Science Graduate School Of Science Hiroshima University
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Suzuki Katsunori
Department Of Biological Science Graduate School Of Science Hiroshima University
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Suzuki Katsunori
Department Of Agricultural Chemistry . Faculty Of Agriculture Nagoya University:(present )biological
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Tanaka Katsuyuki
Department Of Applied Physics Faculty Of Science Tokyo Institute Of Technology
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Matsui Hiroki
Department Of Biological Science Graduate School Of Science Hiroshima University
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Suzuki Katsunori
Dep. Of Biological Sci. Graduate School Of Sci. Hiroshima Univ.
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Tanaka Katsuyuki
Dep. Of Biological Sci. Graduate School Of Sci. Hiroshima Univ.
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