Sequence characterization of the vir region of a nopaline type Ti plasmid, pTi-SAKURA
スポンサーリンク
概要
- 論文の詳細を見る
We isolated a crown gall tumor-inducing nopaline type Ti plasmid from Agrobacterium tumefaciens on a Sakura Japanese cherry tree, and designated it as pTi-SAKURA. By primer walking sequencing with long PCR and a newly developed PCR subcloning technique for long insert DNA, we completed DNA sequencing of the most important functional unit, the virulence (vir) region of pTi-SAKURA, which is indispensable for T-DNA transfer into the plant’s chromosomes. By homology searches with the vir genes of other bacterial plasmids, we identified 11 open reading frames (orfs) and 31 genes and 11 vir box, which are 6 bp regulatory sequences. In total, 26 vir genes, including the putative virF and virK and the main vir region, were present as the vir gene cluster. The presence of vir box, GC content, codon usage and expression analysis in these genes led us to propose a new vir region.
- 日本遺伝学会の論文
著者
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Yoshida K
Department Of Biological Science Graduate School Of Science Hiroshima University
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YOSHIDA Kazuo
Department of Surgery, Shinshu University, School of Medicine
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Hattori Yoshiyuki
Department Of Biological Science Graduate School Of Science Hiroshima University
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Uraji Misugi
Department Of Biological Science Graduate School Of Science Hiroshima University
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Katoh A
Department Of Materials And Life Science Faculty Of Science And Technology Seikei University
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Katoh Akira
Hokkaido Agricultural Experiment Station
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SUZUKI Katsunori
Department of Biological Science, Graduate School of Science, Hiroshima University
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IWATA Kumi
Department of Biological Science, Graduate school of Science, Hiroshima University
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OHTA Nobuyuki
Department of Biological Science, Graduate school of Science, Hiroshima University
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Ohta Nobuyuki
Department Of Biological Science Graduate School Of Science Hiroshima University
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Suzuki Katunori
Department Of Biological Science Graduate School Of Science Hiroshima University
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Iwata Kumi
Department Of Biological Science Graduate School Of Science Hiroshima University
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Yoshida Kazuo
Department Of Biological Science Graduate School Of Science Hiroshima University
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Suzuki Katsunori
Department Of Biological Science Graduate School Of Science Hiroshima University
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Suzuki Katsunori
Department Of Agricultural Chemistry . Faculty Of Agriculture Nagoya University:(present )biological
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Yoshida Kazuo
Department Of Biological Science Faculty Of Science Hiroshima University
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