Roles of sexual cell agglutination in yeast mass mating
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概要
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The agα1 mutant MAT α cells specifically lack the cell surface α-type sexual agglutination substance, which is also called α-agglutinin. Because the mutant cells (MATα ag α1) can not form aggregates with MATa cells, MATα agα1 cells are unable to mate with MATa cells when they are co-inoculated in a liquid medium, and the mating is attenuated on solid medium. The attenuated mating ability shown in the previous studies gave us a vague idea about a physiological function of the sexual agglutinability. In order to solve the question, mating behavior of MATα agα1 cells was investigated here under conditions where the contact between MATa and MAT α cells is assisted by physical methods. A synthetic mutation agα1::URA3 was constructed and used as well as agα1-1 for this study to ensure the genetic defect. When a mixture of MATa and MAT α cells was kept on filter membrane placed on relatively dry agar medium, even agα1::URA3 mutant cells mated as efficiently as the wild type (AGα1) cells did. On filter membrane placed on moist agar medium, agα1 mutants mated 10-fold less efficiently than wild type cells did. The mutant cells mated 10000-time less efficiently than the wild type cells in a pellet formed by brief low speed centrifugation. In contrast, the wild type MATα cells mated well under all conditions tested. Under the pellet condition, a mixture of MATa and MATα AG α1 cells formed an extended and cotton-like pellet while a mixture of MATa and MATα agα1 cells formed a compact and tight pellet. These results suggest that sexual cell agglutination contributes not only to cell contact between MATa and MAT α cells thereby stabilizing a-α cell pairs, but also to construction of a uniquely organized ultra structure favorable for zygote formation and subsequent growth of diploid cells. The mating specific extended pellet formation was observed also in 4 pairs of a and α strains in ascosporogenous yeast genera Hansenula and Pichia.
- 日本遺伝学会の論文
- 2003-06-01
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