Identification of pTi-SAKURA DNA region conferring enhancement of plasmid incompatibility and stability
スポンサーリンク
概要
- 論文の詳細を見る
In Agrobacterium tumefaciens, the stability of Ti plasmids differs depending on the strain. So far, little is known about genes that cause the difference in stability. The repABC operon is responsible for replication and incompatibility of Ti plasmids. We constructed recombinant plasmids carrying the repABC operon and different portions of pTi-SAKURA. Cells having the recombinant plasmids that harbored a 2.6-kbp NheI fragment of pTi-SAKURA were found to be transformed via conjugation 100-fold less frequently with a small incompatible repABC plasmid than cells having the recombinant plasmids lacking the 2.6-kbp NheI fragment. Since the phenomenon occurred only when the resident and incoming plasmids belonged to the same incompatibility group, it was suggested that the 2.6-kbp NheI fragment bears the potential enhancing incompatibility. The fragment contained an operon consisting of two open reading frames, tiorf24 and tiorf25. tiorf24 is an orphan gene, whereas tiorf25 is a homologue of a group of plasmid stability genes. Removal of the 2.6-kbp fragment from the resident pTi-SAKURA increased the resident plasmid ejection ratio by the incoming repABC plasmid, whereas addition of the fragment to pTiC58 decreased the ejection ratio, and the loss ratio during growth at 37°C. These data suggest that tiorf24 and tiorf25 are responsible for the stability of pTi-SAKURA, and reduce, in the host bacterium, the frequency of ejection of the resident plasmid, presumably through an incompatibility mechanism.
- 日本遺伝学会の論文
- 2007-06-25
著者
-
YAMAMOTO Shinji
Department of Neurosurgery, Kyoto Second Red Cross Hospital
-
TANAKA Katsuyuki
Department of Neurosurgery, St. Marianna University School of Medicine, Kawasaki
-
Uraji Misugi
Department Of Biological Science Graduate School Of Science Hiroshima University
-
SUZUKI Katsunori
Department of Biological Science, Graduate School of Science, Hiroshima University
-
MORIGUCHI Kazuki
Department of Biological Science, Graduate School of Science, Hiroshima University
-
Moriguchi Kazuki
Department Of Biological Science Graduate School Of Science Hiroshima University
-
Tanaka Katsuyuki
Department Of Biological Science Graduate School Of Science Hiroshima University
-
Yamamoto Shinji
Department Of Biological Science Graduate School Of Science Hiroshima University
-
Yamamoto Shinji
Department Of Animal Husbandry Faculty Of Agriculture Tohoku University
-
Suzuki Katsunori
Department Of Agricultural Chemistry . Faculty Of Agriculture Nagoya University:(present )biological
-
Tanaka Katsuyuki
Department Of Applied Physics Faculty Of Science Tokyo Institute Of Technology
関連論文
- Percutaneous Vertebroplasty Under Three-Dimensional Radiography Guidance : Technical Note
- 高気圧酸素療法を施した患者の抗がん薬カルボプラチン血漿中濃度測定用HPLC法について
- Lack of Promoting Potential of Dimethylarsinic Acid in the Kidney of Male NCI-Black Reiter Rats
- CYTOCHEMICAL STUDIES ON THE LESION OF EQUINE INFECTIOUS ANEMIA VII. ESPECIALLY ON THE ENZYME HISTOCHEMISTRY OF RES IN THE LIVER AND SPLEEN
- MORPHOLOGICAL STUDIES ON THE SEXUAL MATURATION IN THE MALE JAPANESE QUAIL (COTURNIX COTURNIX JAPONICA) II. THE GERM CELL TYPES AND CELLULAR ASSOCIATIONS DURING SPERMATOGENESIS
- PHYSIO-HISTOLOGICAL STUDIES ON THE PHYSIOLOGICAL OBESITY OF MEAT PIGS, (REPORT IX), ESPECIALLY ON THE OCCURRENCE OF GLYCOGEN WITHIN THE NUCLEI AND CYTOPLASMS OF THE ZONA RETICULARIS IN THE ADRENALS OF WILD BOARS AND LANDRACE MEAT PIGS IN COMPARISON WITH T
- HISTOCHEMICAL DEMONSTRATION OF THE Δ^5-3β-HYDROXYSTEROID DEHYDROGENASE ACTIVITY IN THE ADRENAL GLANDS OF THE DOMESTIC FOWL AND JAPANESE QUAIL
- PHYSIO-HISTOLOGICAL STUDIES ON THE PHYSIOLOGICAL OBESITY OF THE MEAT PIGS, (REPORT VIII) ESPECIALLY ON THE HISTOCHEMICAL OBSERVATIONS OF THE SUTHERLAND'S HYPOTHESIS CONNECTED WITH GLYCOGENOLYSIS, COENZYMES AND STEROID HORMONE AS THE HORMONAL REGULATION OF
- PHYSIO-HISTOLOGICAL STUDIES ON THE PHYSIOLOGICAL OBESITY OF THE MEAT PIGS (REPORT V), ESPECIALLY ON THE DISTRIBUTION OF SUCCINIC DEHYDROGENASE, ALKALINE AND ACID PHOSPHATASES ON THE COURSE OF CORTICOSTEROID PRODUCTION IN THE SWINE ADRENALS
- p53 and H-ras Mutations and Microsatellite Instability in Renal Pelvic Carcinomas of NON/Shi Mice Treated with N-Butyl-N-(4-hydroxybutyl)-nitrosamine: Different Genetic Alteration from Urinary Bladder Carcinoma
- p53 in Multiple Human Urothelial Cancers: Assessment for Clonality by the Yeast p53 Functional Assay in Combination with p53 Immunohistochemistry
- Loss of Heterozygosity in (Lewis×F344)F_1 Rat Urinary Bladder Tumors Induced with N-Butyl-N-(4-hydroxybutyl) nitrosamine Followed by Dimethylarsinic Acid or Sodium L-Ascorbate
- Evaluation of the Effect of Heat Exposure on the Autonomic Nervous System by Heart Rate Variability and Urinary Catecholamines
- Assessing the Influence of Antivibration Glove on Digital Vascular Responses to Acute Hand-arm Vibration
- Body Mass Index Change after One Year from a Health Examination and its Relation to Attitudes toward Lifestyle Change in Japanese Male Workers : Short Communication
- Modulation of Motility and Proliferation of Glioma Cells by Hepatocyte Growth Factor
- Pharmacokinetic Investigation of Increased Efficacy Against Malignant Gliomas of Carboplatin Combined With Hyperbaric Oxygenation
- Radioimmunotherapy of solid tumors targeting a cell-surface protein, FZD10 : therapeutic efficacy largely depends on radiosensitivity
- Isolation of Differentiated Squamous and Undifferentiated Spindle Carcinoma Cell Lines with Differing Metastatic Potential from a 4-Nitroquinoline N-Oxide-induced Tongue Carcinoma in a F344 Rat
- Association of the sea urchin EGF-related peptide, EGIP-D, with fasciclin I-related ECM proteins from the sea urchin Anthocidaris crassispina
- Dexamethasone Increases β_2 : Adrenoceptor : Regulated Phosphatidylcholine Secretion in Rat Alveolar Type II Cells
- Giant Basal Cell Carcinoma Associated with Systemic Amyloidosis
- A novel plasmid curing method using incompatibility of plant pathogenic Ti plasmids in Agrobacterium tumefaciens
- Sequence characterization of the vir region of a nopaline type Ti plasmid, pTi-SAKURA
- STRUCTURE OF TI PLASMID PTI-SAKURA(10) : Characterization of left-and right-side boundary of the vir gene region
- STRUCTURE OF TI PLASMID PTI-SAKURA(9) : Characterization of tra gene region and its boundary
- Identification of pTi-SAKURA DNA region conferring enhancement of plasmid incompatibility and stability
- Self-sterilizing catheters with titanium dioxide photocatalyst thin films for clean intermittent catheterization : Basis and study of clinical use
- Preparation of Polycrystalline Bismuth and Aluminum Substituted Yttrium-Iron Garnet Films by Repetitive Gel Coating on Glass Substrates
- Inhibition of Hydroxyquinol Auto-Oxidation by a Cell Extract of Trichosporon cutaneum
- Precise characterization of rDNA genes by intraspecies and inter-loci comparison of rDNA sequences and biochemical analysis of ribosomal RNA molecules in Agrobacterium tumefaciens
- Differential Light Regulation of the rbcS Gene Expression in Two Cell Lines of the Liverwort Marchantia paleacea var.diptera
- Light-Dependent Expression of Protochlorophyllide Oxidoreductase Gene in the Liverwort, Marchantia paleacea var.diptera
- EXPERIMENTAL STUDIES ON THE AMMONIA POISONING OF THE GOAT I. ON THE CAPACITY TO DETOXICATE AMMONIA ENTERING THROUGH THE PORTAL BLOOD
- P-MC-04 New Method for Evaluation of the Ultra-Thin Air Film Spacing between Head Slider and Disk Surface on Magnetic Disk Files : General Description of Problem and Solving Method
- P-HDI-02 EXAMINATION OF FLYING HEIGHT OF MAGNETIC HEAD SLIDER IN SIMULATIONS AND MEASUREMENTS AT NANOMETER-ORDER SPACING
- Japanese Traditional Printing "Ukiyo-e" in a Virtual Space
- Human Lysozyme Secretion Increased by Alpha-factor Pro-sequence in Pichia pastoris
- A Case of A Non-Insulin Dependent Diabetic Patient with Regular Spontaneous Hypoglycemic Attacks, Which were Due to Insulin-Binding Antibodies Induced by Human Insulin Therapy
- Characterization of a cDNA Encoding CuZn-Superoxide Dismutase from the Liverwort Marchantia paleacea var.diptera
- Purification of the Cytosolic CuZn-Superoxide Dismutase (CuZn-SOD) of Marchantia paleacea var. diptera and its Resemblance to CuZn-SOD from Chloroplasts
- Construction of physical map and mapping of chromosomal virulence genes of the biovar 3 Agrobacterium (Rhizobium vitis) strain K-Ag-1
- Density Inhomogeneity in Amorphous Chlorobenzene Vapor-Deposited on Cold Substrates
- Gene cluster for ferric iron uptake in Agrobacterium tumefaciens MAFF301001
- Proton Nuclear Magnetic Resonance in TaH_
- Genome analysis of Agrobacterium tumefaciens: Linkage map and genetic features of the left region of the linear chromosome
- Traumatic Longitudinal Clival Fracture in a Child
- Behavior of Hydrogen in Ta-H System
- Roles of sexual cell agglutination in yeast mass mating
- Metabolism of p-Hydroxybenzoate via Hydroxyquinol by Trichosporon cutaneum WY2-2 : Characterization of the Pathway using Superoxide Disimnutase as a Stabilizer of Hydroxyquinol
- MORPHOLOGICAL STUDIES ON THE SEXUAL MATURATION IN THE MALE JAPANESE QUAIL (COTURNIX COTURNIX JAPONICA) I. EFFECT OF CONTINOUS LIGHTING AND CONSTANT TEMPERATURE ON THE TESTIS GROWTH
- Inter-observer and intra-observer reliabilities of the Japanese Ministry of Health, Labor and Welfare type classification system for osteonecrosis of the femoral head
- Lack of Promoting Potential of Dimethylarsinic Acid in the Kidney of Male NCI-Black Reiter Rats
- Interstitial pneumonia induced by combined intraarterial 5-fluorouracil and subcutaneous interferon-α therapy for advanced hepatocellular carcinoma
- Adsorption measurements during catalytic isomerization of pentenes and 2-methyl-1-pentene over KC24.
- Surface Tensions of trans-1,3,3,3-Tetrafluoropropene and trans-1,3,3,3-Tetrafluoropropene+Difluoromethane Mixture
- Microscopic evaluation of surfactant-water interaction in apolar media.