Genome analysis of Agrobacterium tumefaciens: Linkage map and genetic features of the left region of the linear chromosome
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概要
- 論文の詳細を見る
In addition to a unique tumor-inducing (Ti) plasmid, the plant pathogenic bacterium Agrobacterium tumefaciens has an unconventional chromosomal organization. Our previous studies on A. tumefaciens MAFF301001 revealed that it possesses a 2 Mb linear and a 2.8 Mb circular chromosome plus a 206.479 kbp Ti plasmid (pTi-SAKURA). In this study, a linkage map for the left half of its linear chromosome covering a 900 kbp region was constructed and the number of potential genes existing in the region was estimated. The linkage map consists of 31 BAC and 8 lambda phage recombinants without any gaps. It confirmed the size and all the structural landmarks indicated in the corresponding region of our previously constructed physical map for the linear chromosome. Sequencing analysis of the end-regions of each linking clone led to the identification of 6 genes and another 27 potential genes or ORFs, including genes and/or gene clusters responsible for homologous recombination (ruvB ), trehalose/maltose sugar transport (thuR, thuG) and alanine catabolism (dadR). Two virulence-related gene homologues (attK and celB), previously reported in the circular chromosome of a different strain of A. tumefaciens were found in this region. These findings will provide a ready-to-use linkage map for further functional analysis of the linear chromosome.
- 日本遺伝学会の論文
- 2001-12-01
著者
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Suzuki Katsunori
Department Of Agricultural Chemistry . Faculty Of Agriculture Nagoya University:(present )biological
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Suzuki Katsunori
Department Of Riological Science Graduate School Of Science Hiroshima University
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DE COSTA
Department of Riological Science, Graduate School of Science, Hiroshima University
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SATOU Megumi
Department of Riological Science, Graduate School of Science, Hiroshima University
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De Costa
Department Of Riological Science Graduate School Of Science Hiroshima University
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Yoshida Kazuo
Department Of Biological Science Faculty Of Science Hiroshima University
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Satou Megumi
Department Of Riological Science Graduate School Of Science Hiroshima University
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