Cytotoxic Synergy between Indomethacin and Hydrochloric Acid in Gastric Mucosal Cells(Biochemistry/Molecular Biology)
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概要
- 論文の詳細を見る
Orally ingested non-steroidal anti-inflammatory drugs (NSAIDs) and acid in gastric secretions are gastric irritants that co-exist at the surface of the gastric mucosa. Here, we examined the individual and combined effects of indomethacin, a typical NSAID, and hydrochloric acid on cell death in primary cultures of guinea pig gastric mucosal cells. Indomethacin alone (at concentrations less than 200μM) did not induce apoptosis; however, hydrochloric acid-induced apoptosis was stimulated in the presence of indomethacin (50-200 μM). Isobologram analysis confirmed the presence of a cytotoxic synergy between indomethacin and hydrochloric acid. The synergistic response between the two gastric irritants was also observed for necrosis. Given that the IC_<50> value of indomethacin for inhibition of prostaglandin synthesis is about 5 nM, the synergistic response between indomethacin and hydrochloric acid appears to be independent of the inhibition of cyclooxygenase activity by indomethacin.
- 社団法人日本薬学会の論文
- 2004-08-01
著者
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土屋 照雄
Gifu Prefectural Hospital
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Tsuchiya Tomofusa
岡山大学 薬学部
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Tsuchiya Tomofusa
Faculty Of Pharmaceutical Sciences Okayama University
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Mizushima Tohru
Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University
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Tanaka K
Department Of Molecular Engineering Graduate School Of Engineering Kyoto University
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Mima Takehiko
岡山大学 医歯薬学総合研究科
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Miki T
Faculty Of Pharmaceutical Sciences Kyushu University
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HOSHINO Tyuji
Department of Physical Chemistry, Graduate School of Pharmaceutical Sciences, Chiba University
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Tsuchiya T
Faculty Of Pharmaceutical Sciences Okayama University
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Tsuchiya T
Okayama Univ. Okayama
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Hoshino T
Department Of Physical Chemistry Graduate School Of Pharmaceutical Sciences Chiba University
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Hoshino Tyuji
Department Of Physical Chemistry Graduate School Of Pharmaceutical Sciences Chiba University
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Tanaka Ken-ichiro
Faculty Of Pharmaceutical Sciences Okayama University
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TOMISATO Wataru
Faculty of Pharmaceutical Sciences, Okayama University
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TSUTSUMI Shinji
Faculty of Pharmaceutical Sciences, Okayama University
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HOSHINO Tatsuya
Faculty of Pharmaceutical Sciences, Okayama University
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MIZUSHIMA Tohru
Faculty of Pharmaceutical Sciences, Okayama University
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Tanaka Katsuyuki
Dept. Biol. Sci. Fac. Sci. Hiroshima Univ.
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Tanaka K
Dept. Biol. Sci. Fac. Sci. Hiroshima Univ.
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Tsuchiya T
Faculty Of Pharmaceutical Sciences Hokuriku University
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Mizushima Tohru
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Mizushima Tohru
Faculty Of Pharmaceutial Sciences Kyushu University
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Mizushima Tohru
Faculty Of Engineering. Tokai University
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Tomisato Wataru
Faculty Of Pharmaceutical Sciences Okayama University
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Tsutsumi S
Faculty Of Pharmaceutical Sciences Okayama University
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Tsuchiya Tomofusa
Department Of Microbiology Fujita Health University
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Tsutsumi Shinji
Faculty Of Pharmaceutical Sciences Okayama University
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Mizushima T
Faculty Of Pharmaceutial Sciences Kyushu University
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Mizushima Tohru
Faculti of Pharmaceutical Sciences Okairama University
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