Functional Gene Cloning and Characterization of MdeA, a Multidrug Efflux Pump from Staphylococcus aureus(Microbiology)
スポンサーリンク
概要
- 論文の詳細を見る
A DNA fragment conferring drug resistance was cloned from the chromosomal DNA of Staphylococcus aureus N315 using a drug hypersensitive Escherichia coli KAM32 as the host. Although E. coli KAM32 cells were sensitive to many antimicrobial agents, transformed cells harboring a recombinant plasmid carrying the DNA region became resistant to several structurally unrelated antimicrobial agents, such as tetraphenylphosphonium chloride, Hoechst 33342 and norfloxacin. These results suggest that the cloned DNA fragment carries a gene(s) encoding a multidrug efflux pump. We partially determined the nucleotide sequence of the cloned DNA and found the mdeA gene within it. The E. coli cells transformed with the mdeA gene showed efflux activity of Hoechst 33342. On the other hand, S. aureus cells transformed with mdeA showed elevated resistance to doxorubicin, daunorubicin, tetraphenylphosphonium chloride, Hoechst 33342, ethidium bromide and rhodamine 6G. Elevated energy-dependent efflux of ethidium was observed with transformed S. aureus. We found that the mdeA gene was expressed under normal growth conditions in S. aureus N315.
- 社団法人日本薬学会の論文
- 2006-04-01
著者
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SHIOTA Sumiko
Laboratory of Pathogenic Microbiology, School of Pharmacy, Shujitsu University
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KURODA Teruo
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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TSUCHIYA Tomofusa
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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Tsuchiya T
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Tsuchiya Tomofusa
岡山大学 分子微生物学
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Kuroda T
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Kuroda Teruo
岡山大学 分子微生物学
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Mizushima Tohru
Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University
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YAMADA Youichi
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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Mima Takehiko
岡山大学 医歯薬学総合研究科
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Yamada Youichi
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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TSUTSUMI Tomoaki
National Institute of Health Sciences
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Tsuchiya Tomofusa
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Kayahara Takashi
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Shimura S
Central Laboratory Lotte Co. Ltd.
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Shiota Sumiko
Laboratory Of Pathogenic Microbiology School Of Pharmacy Shujitsu University
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Kuroda T
Faculty Of Pharmaceutical Sciences Fukuoka University
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Mizushima Tohru
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Mizushima Tohru
Graduate School Of Medical And Pharmaceutical Sciences Kumamoto University
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Kuroda Teruo
Department Of Microbiology Faculty Of Pharmaceutical Science Okayama University
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Tanabe Tomotaka
College Of Pharmaceutical Sciences Matsuyama University
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Kuroda Teruo
Department of Genome Applied Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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