KmrA Multidrug Efflux Pump from Klebsiella pneumoniae(Microbiology)
スポンサーリンク
概要
- 論文の詳細を見る
We cloned a gene responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 that showed multidrug resistance. We designated the gene kmrA. The deduced amino acid sequence of KmrA was similar to that of SmvA that is responsible for methyl viologen-resistance in Salmonella enterica sv. Typhi and Typhimurium. Introduction of the cloned kmrA gene into drug-hypersensitive Escherichia coli KAM32 cells made them resistant to acriflavine, 4',6-diamidino-2-phenylindole (DAPI), Hoechst 33342, tetraphenylphosphonium chloride (TPPCl), methyl viologen and ethidium bromide. We observed elevated energy-dependent efflux of ethidium in E. coli cells carrying the kmrA gene compared with control cells. We also cloned the smvA gene from S. enterica sv. Typhimurium LT2 and investigated the resistance pattern for several drugs. The pattern was similar between KmrA from K. pneumoniae and SmvA from S. enterica.
- 社団法人日本薬学会の論文
- 2006-03-01
著者
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OGAWA Wakano
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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KURODA Teruo
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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TSUCHIYA Tomofusa
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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Tsuchiya T
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Tsuchiya Tomofusa
岡山大学 分子微生物学
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Kuroda T
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Kuroda Teruo
岡山大学 分子微生物学
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Ogawa Wakano
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Ogawa Wakano
岡山大学 分子微生物学
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Ogawa Wakano
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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KOTERASAWA Motohiro
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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TSUTSUMI Tomoaki
National Institute of Health Sciences
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Tsuchiya Tomofusa
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Kayahara Takashi
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Koterasawa Motohiro
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Kuroda T
Faculty Of Pharmaceutical Sciences Fukuoka University
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Kuroda Teruo
Department Of Microbiology Faculty Of Pharmaceutical Science Okayama University
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Tanabe Tomotaka
College Of Pharmaceutical Sciences Matsuyama University
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Kuroda Teruo
Department of Genome Applied Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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