Properties and Expression of a Multidrug Efflux Pump AcrAB-KocC from Klebsiella pneumoniae(Microbiology)
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概要
- 論文の詳細を見る
We previously reported that we had cloned genes responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 using a drug-hypersusceptible Escherichia coll strain as a host. One of the recombinant plasmids pETV6 conferred resistance to host cells against a wide range of antimicrobial agents, dyes and detergents. It was revealed that this plasmid carried the acrB_<Kp> gene and a part of the acrA_<Kp> gene coding for a multidrug efflux pump belonging to the RND family. We cloned the whole acrA_<Kp>B_<Kp> operon of K. pneumoniae and characterized the pump. The AcrAB pump utilized TolC as an outer membrane component in cells of E. coll. Elevated energy-dependent efflux of ethidium was observed with cells possessing AcrA_<Kp> B_<Kp>-TolC. We cloned a gene coding for an ortholog of TolC from chromosomal DNA of K. pneumoniae, and designated it kocC. It seems that the AcrA_<Kp>B_<Kp>-KocC complex functions as a potent multidrug efflux pump in K. pneumoniae. We observed a higher level of expression of acrA_<Kp> in K. pneumoniae MGH78578, a multidrug resistant strain, compared with ATCC10031, a drug susceptible strain.
- 社団法人日本薬学会の論文
- 2008-04-01
著者
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OGAWA Wakano
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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KURODA Teruo
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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TSUCHIYA Tomofusa
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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Tsuchiya T
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Tsuchiya Tomofusa
岡山大学 分子微生物学
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Kuroda T
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Kuroda Teruo
岡山大学 分子微生物学
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Ogawa Wakano
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Ogawa Wakano
岡山大学 分子微生物学
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Ogawa Wakano
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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LI Dai-Wei
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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ONISHI Motoyasu
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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KISHINO Takanori
Department of Genome Applied Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical
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MATSUO Taira
Department of Genome Applied Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical
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Li Dai
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Li Dai-wei
岡山大学 医歯薬学総合研究科分子微生物学教室
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Onishi Motoyasu
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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TSUTSUMI Tomoaki
National Institute of Health Sciences
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Tsuchiya Tomofusa
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Kishino Takanori
Department Of Genome Applied Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical S
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Kayahara Takashi
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Matsuo Taira
Department Of Genome Applied Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical S
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Kuroda T
Faculty Of Pharmaceutical Sciences Fukuoka University
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Kuroda Teruo
Department Of Microbiology Faculty Of Pharmaceutical Science Okayama University
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Tanabe Tomotaka
College Of Pharmaceutical Sciences Matsuyama University
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Kuroda Teruo
Department of Genome Applied Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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