The NhaB Na+/H+ Antiporter Is Essential for Intracellular pH Regulation under Alkaline Conditions in Escherichia coli.
スポンサーリンク
概要
- 論文の詳細を見る
We isolated a mutant of Escherichia coli which was defective in an Na+/H+ antiporter and grew poorly under alkaline conditions [Ishikawa, T., Hama, H., Tsuda, T., and Tsuchiya, T. (1987) J. Biol. Chem. 262, 7443-7446]. Later, it was concluded that the defective Na+/H+ antiporter in the mutant was the NhaB system, and the nhaB gene was mapped to 25.6min on the E. coli chromosome [Thelen, P., Tsuchiya, T., and Goldberg, E. B. (1991) J. Bacteriol. 173, 6553-6557]. We found that the NhaB-defective cells cannot grow in a high pH medium. Furthermore, intracellular pH in the mutant cells was almost the same as extracellular pH between 7.9 and 9.1, that is, intracellular pH was not regulated at this pH range. On the other hand, intracellular pH of the wild-type cells was maintained at about 7.6 when the extracellular pH was between 7.6 and 8.5. Thus, the NhaB Na+/H+ antiporter is essential for the regulation of intracellular pH under alkaline conditions in E. coli. Introduction of nhaA gene into the mutant cells increased Na+/H+ antiporter activity, but did not restore the defective growth and defective intracellular pH regulation under alkaline conditions.
- 社団法人 日本生化学会の論文
著者
-
Tsuchiya Tomofusa
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
-
Tsuda Masaaki
Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceuti
-
Inaba Kei
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
-
Ishikawa Tetsuya
Department Of Applied Chemistry Faculty Of Science Science University Of Tokyo
-
Goldberg Edward
Department of Molecular Biology and Microbiology, Tufts University Medical School
-
Shimamoto Toshi
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University
-
Thelen Paul
Department of Molecular Biology and Microbiology, Tufts University Medical School
関連論文
- Synergistic Effect of Kaempferol Glycosides Purified from Laurus nobilis and Fluoroquinolones on Methicillin-Resistant Staphylococcus aureus(Microbiology)
- PJ-268 Predictors of Angiographic Target Lesion Revascularization within 1 Year after Successful Sirolimus-Eluting Stent (Cypher) Implantation(Restenosis basic/clinical-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-664 Comparison of a Sirolimus-eluting Stent with a Bare Metal Stent in Patients with Diabetes Mellitus Treated with Percutaneous Coronary Intervention(Coronary revascularization, PCI-21 (IHD) PJ112,Poster Session (Japanese),The 70th Anniversary Annual
- PJ-366 Initial Results and Mid-term Outcomes of Sirolimus-eluting Stent in Acute Coronary Syndrome(Coronary revascularization, PCI-17 (IHD) PJ62,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-363 Initial and Mid-term Results of Sirolimus-eluting Stents in Patients with Diffuse Long Coronary Lesions-comparison with Bare Metal Stents(Coronary revascularization, PCI-16 (IHD) PJ61,Poster Session (Japanese),The 70th Anniversary Annual Scientific
- PJ-361 Impact of Sirolimus-eluting Coronary Stent (Cypher) on binary restenosis and target lesion revascularization in the RCA Ostiual Lesion(Coronary revascularization, PCI-16 (IHD) PJ61,Poster Session (Japanese),The 70th Anniversary Annual Scientific Me
- OJ-143 Differential Predictors for Binary Restenosis between Bare Metal Stents and Sirolimus-Eluting Stent (Cypher)(Coronary revascularization, PCI-6 (IHD) OJ24,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Ci
- OJ-011 Mid-term Efficacy of Sirolimus-Eluting Stent in the Treatment of Patients with In-Stent Restenotic Coronary Artery(Coronary revascularization, PCI-4 (IHD) OJ2,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japane
- OJ-480 Early Elevation of Serum lnterleukin-6 and High Sensitive CRP Predicts LV Remodeling in Reperfused Myocardial Infarction(Old Myocardial Infarction/Remodeling 1 (IHD) : OJ59)(Oral Presentation (Japanese))
- Synchrotron X-ray Diffraction Studies of the Incommensurate Phase of a Spin-Peierls System CuGeO_3 in Strong Magnetic Fields(Condensed Matter : Electronic Structure, Electrical, Magnetic and Optical Properties)
- Anti-methicillin Resistant Staphylococcus aureus (MRSA) Compounds Isolated from Laurus nobilis(Microbiology)
- Antimicrobial Activity of Oleanolic Acid from Salvia officinalis and Related Compounds on Vancomycin-Resistant Enterococci (VRE)(Microbiology)
- Potentiation of Antimicrobial Activity of Aminoglycosides by Carnosol from Salvia officinalis(Microbiology)
- Polyphenolic Constituent Structures of Zanthoxylum piperitum Fruit and the Antibacterial Effects of Its Polymeric Procyanidin on Methicillin-Resistant Staphylococcus aureus
- Synergistic Effect of [10]-Gingerol and Aminoglycosides against Vancomycin-Resistant Enterococci (VRE)(Microbiology)
- Remarkable Synergies between Baicalein and Tetracycline, and Baicalein and β-Lactams against Methicillin-Resistant Staphylococcus aureus
- Phenolic Constituents of Licorice. VIII. Structures of Glicophenone and Glicoisoflavanone, and Effects of Licorice Phenolics on Methicillin-Resistant Staphylococcus aureus
- PJ-387 Angiographic outcome after Primary Stenting to ST-Segment Elevation Acute Myocardial Infarction with Sirolimus- versus Paclitaxel-Eluting Stents(PJ065,ACS/AMI (Clinical/Treatment) 3 (IHD),Poster Session (Japanese),The 73rd Annual Scientific Meeting
- PE-373 Early Definite Stent Thrombosis of Drugs-Eluting Stents under the First Chosen Thienopyridine Antiplatelet (Ticlopidine versus Clopidogrel) : A Retrospective non-Randomized Study(PE063,Coronary Revascularization, PCI (DES) 5 (IHD),Poster Session (E
- PE-138 Impact of Paclitaxel-Eluting Stent on Predictors of Target Lesion Revascularization of Sirolimus-Eluting Stent : Retrospective Estimation in 2678 Angiographic Followed-up Lesions(PE023,Coronary Revascularization, PCI (DES) 3 (IHD),Poster Session (E
- Deficiency of forkhead box P3 and cytotoxic T-lymphocyte-associated antigen-4 gene expressions and impaired suppressor function of CD4+CD25+ T cells in patients with autoimmune hepatitis
- The SHAP-hyaluronan complex in serum from patients with chronic liver diseases caused by hepatitis virus infection
- Hard X-ray Photoemission Spectroscopy of Temperature-Induced Valence Transition in EuNi_2(Si_Ge)_2(Condensed Matter : Electronic Structure, Electrical, Magnetic and Optical Properties)
- PE-374 Optimal Electrograms for Thoracic Vein Isolation during Active Firing of The Vein(Arrhythmia, therapy(14)(A),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Properties and Expression of a Multidrug Efflux Pump AcrAB-KocC from Klebsiella pneumoniae(Microbiology)
- Functional Gene Cloning and Characterization of the SsmE Multidrug Efflux Pump from Serratia marcescens(Microbiology)
- Molecular Cloning and Characterization of All RND-Type Efflux Transporters in Vibrio cholerae Non-O1
- Gene Cloning and Characterization of KdeA, a Multidrug Efflux Pump from Klebsiella pneumoniae(Microbiology)
- Molecular Cloning and Characterization of a Multidrug Efflux Pump, SmfY, from Serratia marcescens(Microbiology)
- Functional Gene Cloning and Characterization of MdeA, a Multidrug Efflux Pump from Staphylococcus aureus(Microbiology)
- Gene Cloning and Characterization of SdrM, a Chromosomally-Encoded Multidrug Efflux Pump, from Staphylococcus aureus(Microbiology)
- KmrA Multidrug Efflux Pump from Klebsiella pneumoniae(Microbiology)
- Gene Cloning and Properties of the RND-Type Multidrug Efflux Pumps MexPQ-OpmE and MexMN-OprM from Pseudomonas aeruginosa
- Gene Cloning and Characterization of Four MATE Family Multidrug Efflux Pumps from Vibrio cholerae Non-O1
- Physiological Roles of Three Na^+/H^+ Antiporters in the Halophilic Bacterium Vibrio parahaemolyticus
- Multidrug Resistance in Klebsiella pneumoniae MGH78578 and Cloning of Genes Responsible for the Resistance(Microbiology)
- A Major Li^+ Extrusion System NhaB of Pseudomonas aeruginosa : Comparison with the Major Na^+ Extrusion System NhaP
- OJ-264 Novel Gene Transduction Method with Biodegradable Gelatin Hydrogel into Endothelial Progenitor Cells for Vascular Regeneration(Angiogenesis 2 (M) : OJ31)(Oral Presentation (Japanese))
- Hypoxia Inducible Factor-1α Gene Transduction of Endothelial Progenitor Cells for Vascular Regeneration
- PE-046 Secondary Cardiac Events in 1118 Patients with Old Myocardial Infarction Revascularized by Sirolimus Eluting Stent and Bare Metal Stents(PE008,Coronary Revascularization, PCI (DES) 1 (IHD),Poster Session (English),The 73rd Annual Scientific Meeting
- PJ-580 Impact of Long-term Continuous Nitrates on Secondary Cardiac Events in Revascularized Old Myocardial Infarction(Acute myocardial infarction, clinical(diagnosis/treatment)(11)(IHD),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the J
- OE-248 Early Definite Stent Thrombosis after Successful Stenting with Bare-Metal, Sirolimus- and Paclitaxel-Eluting Stents : A High-Volume Single Center Study(Coronary revascularization, PCI(02)(IHD),Oral Presentation(English),The 72nd Annual Scientific M
- Interference of Nuclear Bragg Scattered X-Rays in X-Ray Interferometer with Large Optical Path Difference
- Time Domain Interferometry in X-Ray Region Using Nuclear Resonant Scattering
- Time-Delayed Interferometry with Nuclear Resonant Scattering of Synchrotron Radiation
- Flux Growth and Characterization of α-^Fe_2O_3 Single Crystals for Nuclear Bragg Scattering Optical Components
- Focusing Properties of a Linear-Phase Bragg-Fresnel Lens
- A Plane Wave Diffraction on an Amplitude-Phase Laue Microinterferometer
- High-Resolution Measurements of Nuclear Bragg Scattering from a Synthetic α-^Fe_2O_3 Crystal
- An X-Ray Phase Plate Using Bragg-Case Diffraction
- Usefulness of a Second Temporary Vena Cava Filter for Preventing Acute Pulmonary Thromboembolism
- Significantly lower incidence of early definite stent thrombosis of drug-eluting stents after unrestricted use in Japan using ticlopidine compared to western countries using clopidogrel : A retrospective comparison with western mega-studies
- Identification and Characterization of Functional Residues in a Na^+/H^+ Antiporter (NhaA) from Escherichia coli by Random Mutagenesis^1
- Hybrid Logistic Characterization of Isometric Twitch Force Curve of Isolated Ferret Right Ventricular Papillary Muscle
- Involvement of Wnt-5a in chondrogenic pattern formation in the chick limb bud
- PJ-678 Immediate and Mid-Term Outcomes of Sirolimus-Eluting Stent and Paclitaxel-Eluting Stent Implantation in Diabetic Patients(PJ114,Coronary Revascularization, PCI (DES) 4 (IHD),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japane
- PE-014 Abnormal Heart Rate Variability Predicts Future Cardiovascular Events in Patients with Normal Heart and Structural Heart Disease(PE003,Autonomic Nervous System (H),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulat
- DPJ-037 Feasibility and Safety on Alternative Site Pacing in Right Ventricle(DPJ07,Arrhythmia, Others (Clinical/Diagnosis/Treatment) 2 (A),Digital Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PJ-444 Safety and Efficacy of Retrograde Approach to Chronic Total Occlusion(Coronary revascularization, PCI(20)(IHD),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Na^+ -coupled Transport of Melibiose in Escherichia coli : Analysis of Mutants with Altered Cation Specificity(MEMBRANE PROTEINS AND TRANSPORT-I)
- Preparation and Characterization of Everted Membrane Vesicles from Cells of Staphylococcus aureus
- Expression of Fas/Fas ligand (FasL) and its involvement in infiltrating lymphocytes in hepatocellular carcinoma (HCC)
- Organic Halides Formation from Humic Substances by Chlorination and Mutagenicity Testing of the Halides (Regular Presentations) (Proceedings of the 9th Symposium on Environmental Pollutants and Toxicology)
- THE EFFECT OF CHEMICALS IN ENVIRONMENT ON AQUATIC ORGANISMS. II, RELATIONS OF SPECIES OF ORGANISMS AND PARTITION-COEFFICIENT OF CHEMICALS TO TOXICITY
- EFFECT OF CHEMICALS IN ENVIRONMENT ON AQUATIC ORGANISMS
- FORMATION OF METALLOTHIONEIN IN FISH
- Switching of Photon Helicities in the Hard X-Ray Region with a Perfect Crystal Phase Retarder
- P406 EFFECTS OF ACIDOSIS ON THE CONTRACTILE PROPERTIES IN FERRET INTACT CARDIAC MUSCLES
- C Terminal Half Fragment (50kDa) of Heavy Chain Components of Clostridium botulinum Type C and D Neurotoxins Can Be Used as an Effective Vaccine
- Hydrophilic Statin Contributes to Therapeutic Vasculogenesis through the Upregulation of Endothelial Progenitor Cells Kinetics(Angiogenesis 2 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Serum-Free Ex-Vivo Expansion of Multipotent Stem Cells Derived from Human Bone Marrow and Cell Transplantation into Animal Models(Transplantation (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Establishment of a Functionally Active Collagen-Binding Vascular Endothelial Growth Factor Fusion Protein(Vasoactive Compound/Growth Factor/Cytokine 1 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Differential Impact of Smoking on Target Lesion Revascularization after Balloon Angioplasty and Stenting to Ischemic Heart Diseases
- Involvement of the Vibrio parahaemolyticus pvsC Gene in Export of the Siderophore Vibrioferrin
- Purification, Reconstitution, and Characterization of Na^+/Serine Symporter, SstT, of Escherichia coli
- Isolation and Characterization of an Escherichia coli Mutant Lacking the Major Serine Transporter, and Cloning of a Serine Transporter Gene
- GENES AFFECTING SERINE-SENSITIVITY IN Escherichia coli
- The Prognostic Predictors in Octogenarian, Eldery Patients Aged over 80 years old, with Acute Myocardial Infarction(AMI) : Multicenter Study of HIJC Group (Acute Myocardial Infarction, Clinical (Diagnosis/Treatment) 6 (IHD), The 69th Annual Scientific Mee
- The Mechanism of Therapeutic Neovascularization using Hypoxia Inducible Factor-1α Gene Transduction of Endothelial Progenitor Cells(Gene Therapy 3 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Thrombopoietin Contributes to Therapeutic Vasculogenesis through Enhanced Antiapoptotic and Migratory Effects on Endothelial Progenitor Cells(Angiogenesis 1 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Therapeutic Vasculogenesis for Myocardial Ischemia by Serum Free Ex Vivo Expanded Endothelial Progenitor Cells from Cord Blood Derived CD133+ Cells(Vascular Regeneration (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Molecular Cloning and Characterization of the HmrM Multidrug Efflux Pump from Haemophilus influenzae Rd
- Gene Cloning and Characterization of VcrM, a Na^+-Coupled Multidrug Efflux Pump, from Vibrio cholerae Non-01
- Multidrug Resistance in Serratia marcescens and Cloning of Genes Responsible for the Resistance
- Functional Cloning and Expression of emeA, and Characterization of EmeA, a Multidrug Efflux Pump from Enterococcus faecalis
- nhaG Na^+/H^+ Antiporter Gene of Bacillus subtilis ATCC9372, Which Is Missing in the Complete Genome Sequence of Strain 168, and Properties of the Antiporter
- Na^+(Li^+)/H^+ Antiporter in Pseudomonas aeruginosa and Effect of Li^+ on Cell Growth
- Mechanisms of Action of Corilagin and Tellimagrandin I That Remarkably Potentiate the Activity of β-Lactams against Methicillin-Resistant Staphylococcus aureus
- Marked Reduction in the Minimum Inhibitory Concentration (MIC) of β-Lactams in Methicillin-Resistant Staphylococcus aureus Produced by Epicatechin Gallate, an Ingredient of Green Tea (Camellia sinensis)
- Phenolic Constituents of Cassia Seeds and Antibacterial Effect of Some Naphthalenes and Anthraquinones on Methicillin-Resistant Staphylococcus aureus
- CHARACTERISTICS OF THE ADENOSINE-FORMYCIN TRANSPORT SYSTEM OF Vibrio parahaemolyticus
- Evidence for Chloramphenicol/H^+ Antiport in Cmr (MdfA) System of Escherichia coli and Properties of the Antiporter
- Valproic acid induces up- or down-regulation of gene expression responsible for the neuronal excitation and inhibition in rat cortical neurons through its epigenetic actions
- Transcriptional Regulation of Neuronal Genes and Its Effect on Neural Functions : Cumulative mRNA Expression of PACAP and BDNF Genes Controlled by Calcium and cAMP Signals in Neurons
- Involvement of endogenous PACAP expression in the activity-dependent survival of mouse cerebellar granule cells
- A case of arteriovenous malformation with neurofibromatosis-1
- Aluminum maltolate inhibits brain-derived neurotrophic factor-induced intracellular free calcium elevations in cultured rat hippocampal neurons (特集 微量元素研究 up-to-date) -- (Neurological Diseases and Trace Elements)
- Errata: Properties and Expression of a Multidrug Efflux Pump AcrAB-KocC from Klebsiella pneumoniae[Biol. Pharm. Bull. 31(4): 577-582 (2008)]
- Position-dependent effect of a neural-restrictive silencer-like element present in the promoter downstream of the SCG10-like protein gene
- The NhaB Na+/H+ Antiporter Is Essential for Intracellular pH Regulation under Alkaline Conditions in Escherichia coli.