Functional Gene Cloning and Characterization of the SsmE Multidrug Efflux Pump from Serratia marcescens(Microbiology)
スポンサーリンク
概要
- 論文の詳細を見る
We cloned a gene responsible for multidrug resistance from chromosomal DNA of Serratia marcescens, and determined the nucleotide sequence. We designated the gene as ssmE. The deduced amino acid sequence of SsmE showed high similarity with the small multidrug resistance (SMR)-type multidrug efflux pumps. Cells of Escherichia coli KAM32, a drug hyper-susceptible mutant, transformed with a plasmid pESM437 carrying the ssmE gene showed elevated minimum inhibitory concentrations of structurally unrelated antimicrobial agents. E. coli KAM32/pESM437 showed elevated energy dependent efflux of ethidium. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that ssmE was expressed in cells of S. marcescens.
- 社団法人日本薬学会の論文
- 2008-03-01
著者
-
OGAWA Wakano
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
-
KURODA Teruo
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
-
TSUCHIYA Tomofusa
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
-
Tsuchiya T
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
-
Tsuchiya Tomofusa
岡山大学 分子微生物学
-
Kuroda T
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
-
Kuroda Teruo
岡山大学 分子微生物学
-
Ogawa Wakano
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
-
Ogawa Wakano
岡山大学 分子微生物学
-
Ogawa Wakano
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
-
MINATO Yusuke
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
-
SHAHCHERAGHI Fereshteh
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
-
TSUTSUMI Tomoaki
National Institute of Health Sciences
-
Tsuchiya Tomofusa
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
-
Morita Y
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
-
Kayahara Takashi
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
-
Minato Yusuke
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
-
Kuroda T
Faculty Of Pharmaceutical Sciences Fukuoka University
-
Minato Yusuke
Department Of Biosciences And Informatics Faculty Of Science And Technology Keio University
-
Kuroda Teruo
Department Of Microbiology Faculty Of Pharmaceutical Science Okayama University
-
Tanabe Tomotaka
College Of Pharmaceutical Sciences Matsuyama University
-
Kuroda Teruo
Department of Genome Applied Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
-
Shahcheraghi Fereshteh
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
関連論文
- Synergistic Effect of Kaempferol Glycosides Purified from Laurus nobilis and Fluoroquinolones on Methicillin-Resistant Staphylococcus aureus(Microbiology)
- スルメイカおよびサンマの可食部位別におけるダイオキシン類汚染レベル
- Synthetic Studies on Aphidicolane and Stemodane Diterpenes. III.An Alternative Stereoselective Access to Aphidicolane-Type B/C/D-Ring System
- 61 アフィディコラン型およびステモダン型ジテルペンの合成研究(ポスター発表の部)
- Gene Cloning and Characterization of EfmA, a Multidrug Efflux Pump, from Enterococcus faecium(Microbiology)
- Synthetic Studies on Aphidicolane and Stemodane Diterpenes. II. Neighboring Hydroxyl Group Participation in Stereoselective Syntheses of Tricyclo[6.3.1.0^]dodecanes Corresponding to the B/C/D-Ring Systems
- Anti-methicillin Resistant Staphylococcus aureus (MRSA) Compounds Isolated from Laurus nobilis(Microbiology)
- Antimicrobial Activity of Oleanolic Acid from Salvia officinalis and Related Compounds on Vancomycin-Resistant Enterococci (VRE)(Microbiology)
- Potentiation of Antimicrobial Activity of Aminoglycosides by Carnosol from Salvia officinalis(Microbiology)
- Polyphenolic Constituent Structures of Zanthoxylum piperitum Fruit and the Antibacterial Effects of Its Polymeric Procyanidin on Methicillin-Resistant Staphylococcus aureus
- Synergistic Effect of [10]-Gingerol and Aminoglycosides against Vancomycin-Resistant Enterococci (VRE)(Microbiology)
- Interaction of the Aryl Hydrocarbon Receptor with Several Constituents from Spinach and Komatsuna Extracts Determined Using in Vitro Bioassay
- Remarkable Synergies between Baicalein and Tetracycline, and Baicalein and β-Lactams against Methicillin-Resistant Staphylococcus aureus
- Phenolic Constituents of Licorice. VIII. Structures of Glicophenone and Glicoisoflavanone, and Effects of Licorice Phenolics on Methicillin-Resistant Staphylococcus aureus
- Protein crystallization in microgravity
- Protein Crystallization in Microgravity (特集:日本の宇宙生物学実験)
- Properties and Expression of a Multidrug Efflux Pump AcrAB-KocC from Klebsiella pneumoniae(Microbiology)
- Functional Gene Cloning and Characterization of the SsmE Multidrug Efflux Pump from Serratia marcescens(Microbiology)
- Molecular Cloning and Characterization of All RND-Type Efflux Transporters in Vibrio cholerae Non-O1
- Gene Cloning and Characterization of KdeA, a Multidrug Efflux Pump from Klebsiella pneumoniae(Microbiology)
- Molecular Cloning and Characterization of a Multidrug Efflux Pump, SmfY, from Serratia marcescens(Microbiology)
- Functional Gene Cloning and Characterization of MdeA, a Multidrug Efflux Pump from Staphylococcus aureus(Microbiology)
- Gene Cloning and Characterization of SdrM, a Chromosomally-Encoded Multidrug Efflux Pump, from Staphylococcus aureus(Microbiology)
- KmrA Multidrug Efflux Pump from Klebsiella pneumoniae(Microbiology)
- Gene Cloning and Properties of the RND-Type Multidrug Efflux Pumps MexPQ-OpmE and MexMN-OprM from Pseudomonas aeruginosa
- Gene Cloning and Characterization of Four MATE Family Multidrug Efflux Pumps from Vibrio cholerae Non-O1
- Physiological Roles of Three Na^+/H^+ Antiporters in the Halophilic Bacterium Vibrio parahaemolyticus
- Multidrug Resistance in Klebsiella pneumoniae MGH78578 and Cloning of Genes Responsible for the Resistance(Microbiology)
- A Major Li^+ Extrusion System NhaB of Pseudomonas aeruginosa : Comparison with the Major Na^+ Extrusion System NhaP
- Activation of the Aryl Hydrocarbon Receptor by Some Vegetable Constituents Determined Using in Vitro Reporter Gene Assay
- Preliminary Screening of the lnhibitory Effect of Food Extracts on Activation of the Aryl Hydrocarbon Receptor Induced by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin
- Identification and Characterization of Functional Residues in a Na^+/H^+ Antiporter (NhaA) from Escherichia coli by Random Mutagenesis^1
- Dioxin Concentrations in Commercial Health Tea Materials in Japan
- 緑葉野菜中のダイオキシン類測定における前処理法の改良と多層シリカゲルカラムの充てん法の比較
- 日本におけるファーストフード中のダイオキシン類レベルとその同族体分布
- 食品中のダイオキシン類測定の前処理における硫酸処理法と多層シリカゲルカラムクロマトグラフ法の比較
- Na^+ -coupled Transport of Melibiose in Escherichia coli : Analysis of Mutants with Altered Cation Specificity(MEMBRANE PROTEINS AND TRANSPORT-I)
- Preparation and Characterization of Everted Membrane Vesicles from Cells of Staphylococcus aureus
- C Terminal Half Fragment (50kDa) of Heavy Chain Components of Clostridium botulinum Type C and D Neurotoxins Can Be Used as an Effective Vaccine
- 日本における市販生鮮および冷凍野菜中のPCDDs,PCDFsおよびコプラナーPCBsレベルと各同族体分布
- Screening of the Inhibitory Effect of Vegetable Constituents on the Aryl Hydrocarbon Receptor-Mediated Activity Induced by 2,3,7,8-Tetrachlorodibenzo-p-dioxin (Miscellaneous)
- プレパック多層シリカゲルカラムを用いた前処理による食品中のダイオキシン類分析
- Involvement of the Vibrio parahaemolyticus pvsC Gene in Export of the Siderophore Vibrioferrin
- Purification, Reconstitution, and Characterization of Na^+/Serine Symporter, SstT, of Escherichia coli
- Isolation and Characterization of an Escherichia coli Mutant Lacking the Major Serine Transporter, and Cloning of a Serine Transporter Gene
- GENES AFFECTING SERINE-SENSITIVITY IN Escherichia coli
- 16 Spirovetivane型Phytoalexin類の合成研究 : (±)-Lubiminolおよび(±)-oxylubiminの立体選択的全合成
- Synthetic Studies on Spirovetivane Phytoalexins. V. : A Stereoselective Total Synthesis of (±)-Oxylubimin
- Determination of Valproic Acid in Human Serum by High-Performance Liquid Chromatography with Fluorescence Detection
- 6, 7-Methylenedioxy-1-methyl-2-oxo-1, 2-dihydroquinoxalin-3-ylpropionohydrazide as a Highly Sensitive Fluorescence Derivatization Reagent for Carboxylic Acids in High-Performanse Liquid Chromatography
- Molecular Cloning and Characterization of the HmrM Multidrug Efflux Pump from Haemophilus influenzae Rd
- Gene Cloning and Characterization of VcrM, a Na^+-Coupled Multidrug Efflux Pump, from Vibrio cholerae Non-01
- Multidrug Resistance in Serratia marcescens and Cloning of Genes Responsible for the Resistance
- Functional Cloning and Expression of emeA, and Characterization of EmeA, a Multidrug Efflux Pump from Enterococcus faecalis
- nhaG Na^+/H^+ Antiporter Gene of Bacillus subtilis ATCC9372, Which Is Missing in the Complete Genome Sequence of Strain 168, and Properties of the Antiporter
- Na^+(Li^+)/H^+ Antiporter in Pseudomonas aeruginosa and Effect of Li^+ on Cell Growth
- Mechanisms of Action of Corilagin and Tellimagrandin I That Remarkably Potentiate the Activity of β-Lactams against Methicillin-Resistant Staphylococcus aureus
- Marked Reduction in the Minimum Inhibitory Concentration (MIC) of β-Lactams in Methicillin-Resistant Staphylococcus aureus Produced by Epicatechin Gallate, an Ingredient of Green Tea (Camellia sinensis)
- 調理による小松菜中のポリ塩化ダイオキシン及びその関連化合物の消長
- Phenolic Constituents of Cassia Seeds and Antibacterial Effect of Some Naphthalenes and Anthraquinones on Methicillin-Resistant Staphylococcus aureus
- High-Performance Liquid Chromatographic Quantification of Busulfan in Human Serum after Fluorescence Derivatization by 2-Naphthalenethiol
- Determination of Phenytoin and Its Major Metabolites in Human Serum by High-Performance Liquid Chromatography with Fluorescence Detection
- Elecrochemical Stability of Desmopressin Acetate during Iontophoresis
- Effect of Pulse on Iontophoretic Delivery of Desmopressin Acetate in Rats
- CHARACTERISTICS OF THE ADENOSINE-FORMYCIN TRANSPORT SYSTEM OF Vibrio parahaemolyticus
- MECHANISM OF GROWTH INHIBITION OF E. COLI BY SERINE AND GENES INVOLVED IN THE SERINE SENSITIVITY
- Identification of Genes, desR and desA, Required for Utilization of Desferrioxamine B as a Xenosiderophore in Vibrio furnissii
- Identification and Characterization of Mouse Type II Platelet-Derived Growth Factor Receptor α Transcript
- Evidence for Chloramphenicol/H^+ Antiport in Cmr (MdfA) System of Escherichia coli and Properties of the Antiporter
- Identification and Characterization of Genes Required for Utilization of Desferri-Ferrichrome and Aerobactin in Vibrio parahaemolyticus(Highlighted paper selected by Editor-in-chief,Microbiology)
- The Protein Encoded by NCgl1221 in Corynebacterium glutamicum Functions as a Mechanosensitive Channel
- Errata: Properties and Expression of a Multidrug Efflux Pump AcrAB-KocC from Klebsiella pneumoniae[Biol. Pharm. Bull. 31(4): 577-582 (2008)]
- Interaction of Some Plant Food Extracts with Aryl Hydrocarbon Receptor Determined by in Vitro Reporter Gene Assay
- Identification and characterization of a Vibrio mimicus gene encoding the heme/hemoglobin receptor
- Identification and Characterization of an Outer Membrane Receptor Gene in Acinetobacter baumannii Required for Utilization of Desferricoprogen, Rhodotorulic Acid, and Desferrioxamine B as Xenosiderophores
- The NhaB Na+/H+ Antiporter Is Essential for Intracellular pH Regulation under Alkaline Conditions in Escherichia coli.
- Characterization of a Gene Encoding the Outer Membrane Receptor for Ferric Enterobactin in Aeromonas hydrophila ATCC 7966^T