Determination of Valproic Acid in Human Serum by High-Performance Liquid Chromatography with Fluorescence Detection
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概要
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A simple and highly sensitive high-performance liquid chromatographic method is described for the determination of valproic acid in human serum. The method is based on the direct derivatization of serum sample with 6,7-methylenedioxy-1-methyl-2-oxo-1,2-dihydroquinoxaline-3-ylpropionohydrazide. The derivatization reaction proceeds in aqueous solution in the presence of pyridine and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide at 37℃. The resulting derivatives were separated on a reversed-phase column (YMC Pack ODS-A) with isocratic elution and were fluorimetrically detected at 440 nm with excitation at 365 nm. The detection limit (signal-to-noise ratio=3) for valproic acid added to serum sample was 0.1 μg (700 fmol)/ml serum sample (2.3 fmol on column). The method was applied to determine the unbound- and total-valproic acid levels in the serum obtained from three healthy volunteers after oral administration of the drug (600 mg).
- 公益社団法人日本薬学会の論文
- 1999-09-15
著者
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Hara Shuuji
Faculty of Pharmaceutical Sciences, Fukuoka University
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INOUE Kazuo
Faculty of Science and Technology,Ryukoku University
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Hara S
Faculty Of Pharmaceutical Sciences Fukuoka University
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Hara Shuuji
Faculty Of Pharmaceutical Sciences Fukuoka University
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Inoue K
Tokyo Research Laboratories Kowa Co. Ltd.
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Kuroda Takeshi
Faculty Of Pharmaceutical Sciences Fukuoka University
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Inoue K
Gifu Pharmaceutical University
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Inoue K
Department Of Pharmacology Kobe Pharmaceutical University
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KAMURA Motoki
Faculty of Pharmaceutical Sciences, Fukuoka University
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FUKUZAWA Misa
Faculty of Pharmaceutical Sciences, Fukuoka University
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ONO Nobufumi
Faculty of Pharmaceutical Sciences, Fukuoka University
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Ono Nobufumi
Faculty Of Pharmaceutical Sciences Fukuoka University
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Kamura Motoki
Faculty Of Pharmaceutical Sciences Fukuoka University
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Fukuzawa Misa
Faculty Of Pharmaceutical Sciences Fukuoka University
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Kuroda T
Faculty Of Pharmaceutical Sciences Fukuoka University
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井上 勝央
名古屋市立大学大学院薬学研究科薬物動態制御学分野
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Inoue Kazuo
Faculty Of Engineering Kansai University
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