Glycerol Uptake in HCT-15 Human Colon Cancer Cell Line by Na^+-Dependent Carrier-Mediated Transport(Biopharmacy)
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概要
- 論文の詳細を見る
It has recently been suggested that an Na^+ -dependent carrier-mediated transport system is involved in intestinal glycerol absorption. Such a transport system is of general interest as a possible pathway of drug delivery and a target of drug development. However, the Na^+ -dependent mechanism of cellular glycerol uptake has not been fully clarified in the small intestine or in any other organ. The purpose of the present study was to examine glycerol uptake in the HCT-15 human colon cancer cell line, which was found to be able to perform Na^+ -dependent glycerol uptake, to determine the transport characteristics and help identify such glycerol transport systems. The uptake of glycerol in HCT-15 cells was highly saturable with a Michaelis constant of 15.0μM and a maximum uptake rate of 11.9pmol/min/mg protein, accompanied by minimal unsaturable transport; it was reduced markedly under Na^+ -free conditions, indicating Na^+ requirement. Glycerol uptake was also reduced by 2, 4-dinitrophenol, a metabolic inhibitor. These results suggest that a carrier-mediated glycerol transport system, which is Na^+ -dependent and secondarily active, is present in HCT-15 cells. The transport system could be specific for glycerol and some analogous compounds with hydroxyl groups, since glycerol uptake was inhibited by some alcohols and compounds related to glycerol, such as 1, 2-propanediol and glycerol 3-phosphpate. However, it may represent a high affinity transport system, which is different from the one in the small intestine, because the Michaelis constant of 15.0μM is about 50-fold lower than that observed in the rat small intestine. In conclusion, this is the first study to demonstrate an Na^+ -dependent carrier-mediated glycerol transport in an established cell line. This will help in identifying a group of Na^+ -dependent glycerol transport systems and elucidating their transport mechanisms, although the one found in HCT-15 cells in this study seems to be different from one previously found in the rat small intestine.
- 社団法人日本薬学会の論文
- 2006-01-01
著者
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Hayashi Yoshie
Department Of Pharmacy Kyoto University Hospital Faculty Of Medicine Kyoto University
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Hayashi Yoshie
京都大学医学部附属病院 薬剤
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林 弥生
College Of Pharmacy Kinjo Gakuin University
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INOUE Katsuhisa
Graduate School of Pharmaceutical Sciences, Nagoya City University
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YUASA Hiroaki
Graduate School of Pharmaceutical Sciences, Nagoya City University
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HAYASHI Yayoi
Graduate School of Pharmaceutical Sciences, Nagoya City University
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FUJIMOTO Nami
Graduate School of Pharmaceutical Sciences, Nagoya City University
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Inoue K
Gifu Pharmaceutical University
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Huang Ye
Department Of Pharmacy Kyoto University Hospital Faculty Of Medicine Kyoto University
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Itoh Kunihiko
Department Of Clinical Pharmacology And Genetics School Of Pharmaceutical Sciences University Of Shi
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Yuasa H
Department Of Pharmaceutics And Drug Delivery School Of Pharmacy Tokyo University Of Pharmacy And Li
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Fujimoto Nami
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Hayashi Yayoi
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Yuasa Hiroaki
Department Of Biopharmaceutics Graduate School Of Pharmaceutical Sciences Nagoya City University
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Yuasa Hiroaki
Graduate School Of Pharmaceutical Sciences Nagoya City University
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井上 勝央
名古屋市立大学大学院薬学研究科薬物動態制御学分野
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Hayashi Y
Life Science Laboratories Central Research Laboratories Ajinomoto Co. Inc.:pharmaceuticals Research
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Hayashi Yayoi
Department Of Biopharmaceutics Faculty Of Pharmaceutical Sciences Nagoya City University
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Inoue Katsuhisa
Graduate School Of Pharmaceutical Sciences Nagoya City University
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