Uptake Mechanism of Fractionated [^3H]Heparin in Isolated Rat Kupffer Cells : Involvement of Scavenger Receptors
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概要
- 論文の詳細を見る
The uptake of fractionated [^3H]heparin was examined to elucidate the uptake mechanism in isolated rat Kupffer cells. The equilibrium binding of fractionated [^3H]heparin to Kupffer cells was concentration-dependent with the dissociation constant of 5.7 nM and the maximum binding capacity of 1.5 pmol/10^6 cells. Several ligands of scaveger receptors inhibited the binding of fractionated [^3H]heparin to Kupffer cells competitively and also the internalization of heparin, suggesting the involvement of scavenger receptors in the uptake of fractionated [^3H]heparin. Fractionated [^3H]heparin was also suggested to be internalized according to first order kinetics with the apparent internalization rate constant of 0.010 min^<-1>. Lowering temperature from 37 to 4℃ reduced the fraction internalized from 33% to 6% without affecting the total association, while the fraction internalized at 25℃ was comparable with that at 37℃. Metabolic inhibitors (2,4-dinitrophenol and rotenone), an inhibitor of receptor-mediated and adsorptive endocytosis of polypeptides (phenylarsine oxide) and phagocytosis inhibitors (cytochalasine B and colchicine) did not inhibit the internalization of fractionated [^3H]heparin. As known inhibitors of receptor-mediated and adsorptive endocytosis of polypeptides and phagocytosis did not affect the uptake of fractionated heparin, the scavenger receptor-mediated uptake is suggested to be ATP-independent and differene from receptor-mediated and adsorptive endocytosis of polypeptides and phagocytosis, although for temperature dependency it showed the typical characteristics of receptor-mediated endocytosis.
- 社団法人日本薬学会の論文
- 1996-04-15
著者
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WATANABE Jun
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine
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林 弥生
College Of Pharmacy Kinjo Gakuin University
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Watanabe J
School Of Pharmacy Aichi Gakuin University
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YUASA HIROAKI
Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Nagoya City University
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HABA Masami
名古屋市立大学薬学部薬剤学教室
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URANO Kimihiko
Department of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Nagoya City University
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HABA Masami
Department of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Nagoya City University
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浦野 公彦
名古屋市立大学薬学部薬剤学教室
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浦野 公彦
浜松医大医病院薬剤部
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Haba M
名古屋市立大学薬学部薬剤学教室
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Yuasa Hiroaki
Department Of Biopharmaceutics Graduate School Of Pharmaceutical Sciences Nagoya City University
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浦野 公彦
School Of Pharmacy Aichi Gakuin University
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Watanabe J
Department Of Pharmaceutics College Of Pharmacy Nihon University
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Watanabe Jun
Department Of Anatomy & Cell Science Kansai Medical University
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Hayashi Yayoi
Department Of Biopharmaceutics Faculty Of Pharmaceutical Sciences Nagoya City University
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