Anticancer Activity of Free γ-Linolenic Acid on AH-109A Rat Hepatoma Cells and the Effect of Serum Albumin on Anticancer Activity of γ-Linolenic Acid in Vitro

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概要

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• The cytotoxicity of γ-linolenic acid (C18 : 3n-6) against rat hepatoma AH-109A cells and the effect of bovine serum albumin (BSA) on its toxicity were examined in culture. The proliferation of AH-109A cells, evaluated by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, was significantly suppressed by γ-linolenic acid above 5 μg/ml concentration in a serum-free culture medium. However, its toxicity was reduced by supplement of BSA. Similar observation of reduced toxicity by BSA was shown by the method of trypan blue dye exclusion and a colony formation assay. The cytotoxicity of γ-linolenic acid was correlated closely with the concentration of unbound (free) γ-linolenic acid. Production of thiobarbituric acid reactive material, one of the indicators of lipid peroxidation, was stimulated by γ-linolenic acid and inhibited by BSA. These results suggested that the presence of albumin suppressed the cytotoxicity of the free fatty acid.

• 公益社団法人日本薬学会の論文

著者

• Hayashi Yoshie

  Department Of Pharmacy Kyoto University Hospital Faculty Of Medicine Kyoto University

• Hayashi Yoshie

  京都大学医学部附属病院 薬剤

• FUKUSHIMA Shoji

  Department of Pathology, Osaka City University Medical School

• KISHIMOTO Shuichi

  Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Kobe Gakuin University

• NAKANO Masahiro

  Department of Physics, Kyoto University

• Kishimoto Shuichi

  Department Of Pharmacy Kumamoto University Hospital:department Of Microbiology Kumamoto University M

• Kishimoto Shuichi

  Department Of Pharmaceutics Faculty Of Pharmaceutical Sciences Kobe Gakuin University

• Nakano M

  Department Of Information Science University Of Occupational And Environmental Health

• Fukushima Shoji

  Faculty Of Pharmaceutical Sciences Kobe-gakuin University

• HAYASHI Yoshiki

  Department of Pharmacy, Kumamoto University Hospital

• HIRATA Takahiro

  Department of Pharmacy, Kumamoto University Hospital

• KATSUKI Takato

  Department of Pharmacy, Kumamoto University Hospital

• Nakano M

  Department Of Biologicalsciences Teikyo University Of Science And Technology

• Fukushima Shoji

  Department Of Pharmacy Kumamoto University Hospital

• Nakano Masahiro

  Department Of Biologicalsciences Teikyo University Of Science And Technology

• 川口 健夫

  城西大学

• Huang Ye

  Department Of Pharmacy Kyoto University Hospital Faculty Of Medicine Kyoto University

• Hayashi Yoshiki

  Department Of Hematology Osaka City General Hospital

• Fukushima Shoji

  Department Of Pathology Osaka City University Medical School

• Hayashi Y

  Life Science Laboratories Central Research Laboratories Ajinomoto Co. Inc.:pharmaceuticals Research

• Hirata T

  Kyowa Hakko Kogyo Co. Ltd. Shizuoka Jpn

• Hirata Takahiro

  Department Of Cell Membrane Biology Institute Of Scientific And Industrial Research Osaka University

• Hayashi Yoshiki

  Department For The Promotion Of General Education And Liberal Arts Kyoto University

• Nakano Masahiro

  Deparment Of Physics Kyushu University

• Fukushima Shoji

  Department Of Gastroenterological Surgery Osaka City University Graduate School Of Medicine

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