Carrier-Mediated Transport of Glycerol in the Perfused Rat Small Intestine(Biopharmacy)
スポンサーリンク
概要
- 論文の詳細を見る
Studies using the closed loop and everted sacs of the rat small intestine recently prompted us to suggest that carrier-mediated transport is involved in the intestinal absorption of glycerol. Although it could be mediated by a novel carrier system, little information is available. The aim of the present study was to kinetically characterize carrier-mediated glycerol transport in the perfused rat small intestine to help in identifying the carrier involved and to explore the possibility that the carrier might be used as a pathway for oral drug delivery and a target for drug development. In situ single-pass per fusion was conducted using a 10-cm midgut segment of the male Wistar rat, and the absorption of [^3H] glycerol was evaluated by its disappearance from the intestinal lumen. The absorption of glycerol was saturable and significantly reduced by removing Na^+ from the per fusion solution, suggesting the involvement of a Na^+ -dependent carrier-mediated transport system. The concentration-dependent absorption profile was successfully analyzed by assuming Michaelis-Menten type carrier-mediated transport and simultaneous passive (diffusive) transport. The maximum transport rate (J_<max>) was 77.0 pmol/s/cm^2 and the Michaelis constant (K_m) was 1.04 mM, giving a J_<max>/K_m of 7.39×10^<-5> cm/s. The membrane permeability coefficient for passive transport (P_<m,d>) was 6.89×10^<-5>cm/s, slightly smaller than J_<max>/K_m. Therefore, it could be the major mechanism of intestinal glycerol absorption in the low concentration range where carrier-mediated transport conforms to linear kinetics represented by J_<max>/K_m. Furthermore, carrier-mediated glycerol transport was found to be inhibited by glycerol 3-phosphate, monoacetin and diglycerol, indicating that the carrier system may be shared by these structural analogues. Thus, the present study has successfully demonstrated and characterized carrier-mediated glycerol transport in the per fused rat small intestine which is a physiologically relevant model.
- 社団法人日本薬学会の論文
- 2006-04-01
著者
-
Hayashi Yoshie
Department Of Pharmacy Kyoto University Hospital Faculty Of Medicine Kyoto University
-
Hayashi Yoshie
京都大学医学部附属病院 薬剤
-
林 弥生
College Of Pharmacy Kinjo Gakuin University
-
OHTA Kin-ya
Graduate School of Pharmaceutical Sciences, Nagoya City University
-
INOUE Katsuhisa
Graduate School of Pharmaceutical Sciences, Nagoya City University
-
YUASA Hiroaki
Graduate School of Pharmaceutical Sciences, Nagoya City University
-
HAYASHI Yayoi
Graduate School of Pharmaceutical Sciences, Nagoya City University
-
Inoue K
Gifu Pharmaceutical University
-
Huang Ye
Department Of Pharmacy Kyoto University Hospital Faculty Of Medicine Kyoto University
-
Ohta Kin-ya
Department Of Biopharmaceutics Graduate School Of Pharmaceutical Sciences Nagoya City University
-
Itoh Kunihiko
Department Of Clinical Pharmacology And Genetics School Of Pharmaceutical Sciences University Of Shi
-
Yuasa H
Department Of Pharmaceutics And Drug Delivery School Of Pharmacy Tokyo University Of Pharmacy And Li
-
Hayashi Yayoi
Graduate School Of Pharmaceutical Sciences Nagoya City University
-
Yuasa Hiroaki
Department Of Biopharmaceutics Graduate School Of Pharmaceutical Sciences Nagoya City University
-
Yuasa Hiroaki
Graduate School Of Pharmaceutical Sciences Nagoya City University
-
井上 勝央
名古屋市立大学大学院薬学研究科薬物動態制御学分野
-
Hayashi Y
Life Science Laboratories Central Research Laboratories Ajinomoto Co. Inc.:pharmaceuticals Research
-
Hayashi Yayoi
Department Of Biopharmaceutics Faculty Of Pharmaceutical Sciences Nagoya City University
-
Inoue Katsuhisa
Graduate School Of Pharmaceutical Sciences Nagoya City University
関連論文
- 01P1-144 ラットにおける経口投与後のnifedipineの消化管吸収に及ぼすカゼイ菌の影響(薬物相互作用(基礎と臨床),医療薬学の扉は開かれた)
- PCFT/SLC46A1による葉酸の吸収機構(ビタミン・バイオファクターのトランスポーター[II])
- Studies on Monoterpene Glucosides and Related Natural Products. XXIII. Biosynthesis of the Secoiridoid Glucosides, Gentiopicroside, Morroniside, Oleuropein, and Jasminin
- 42 イリドイド配糖体の酸化順序とloganinのセコイリドイド配糖体への環開裂の機構
- ラット腸管におけるビオチン輸送担体の機能解析
- Ca 拮抗剤アムロジピンの患者における Population Pharmacokinetics
- Studies on the Blue Pigments Produced from Genipin and Methylamine. II .On the Formation Mechanisms of Brownish-Red Intermediates Leading to the Blue Pigment Formation
- Studies on the Blue Pigments Produced from Genipin and Methylamine. I. Structures of the Brownish-Red Pigments, Intermediates Leading to the Blue Pigments
- 75 Genipinとアミノ酸より生成するPseudoazulene骨格をもつ青色々素の構造に関する研究
- Functional Characterization of Human Aquaporin 9 as a Facilitative Glycerol Carrier
- Absorption and Metabolic Extraction of Diltiazem from the Perfused Rat Small Intestine
- Saturable Absorption of Glycerol in the Rat Intestine(Biopharmacy)
- Kinetic Characterization of Carrier-Mediated Transport Systems for D-Glucose and Taurocholate in the Everted Sacs of the Rat Colon
- Search for Carrier-Mediated Transport Systems in the Rat Colon
- Effect of Maleylated Bovine Serum Albumin on the Disposition of Fractionated Heparin in Rats
- Uptake of FH by Two Types of Scavenger-Like Receptors in Rat Liver Parenchymal Cells in Primary Culture
- Uptake of Fractionated Heparin by Two Types of Scavenger Receptors in Isolated Rat Kupffer Cells
- Scavenger Receptor-Mediated Uptake of Fractionated [^3H] Heparin in Rat Peritoneal Macrophages
- First-Pass Metabolism of 5-Fluorouracil in the Perfused Rat Small Intestine
- Kinetic Characterization of Binding and Internalization of Fractionated [^3H]Heparin in Rat Liver Parenchymal Cells in Primary Culture
- Dose-Dependent Gastrointestinal Absorption of 5-Fluorouracil in Rats in Vivo
- Macromolecule-Macromolecule Interaction in Drug Distribution. V. Effects of Plasma Proteins on Uptake of Fractionated [^3H]Heparin in Isolated Rat Kupffer Cells
- Molecular Weight Dependency in the Uptake of Fractionated [^3H]Heparin in Isolated Rat Kupffer Cells
- Uptake Mechanism of Fractionated [^3H]Heparin in Isolated Rat Kupffer Cells : Involvement of Scavenger Receptors
- Macromolecule-Macromolecule Interaction in Drug Distribution. IV. Molecular Weight Dependency in the Interaction of Fractionated [^3H] Heparin with Plasma Proteins
- INTER-ORGAN RELATION BETWEEN SALIVARY GLAND AND KIDNEY IN LITHIUM EXCRETION UNDER CONTINUOUS STIMULATION OF SALIVATION IN DOGS : EFFECTS OF ORAL ADMINISTRATION OF NaCl OR KCl AND I.V. ADMINISTRATION OF SEVERAL DIURETICS ON LITHIUM CLEARANCES
- Inter-organ Relation between Salivary Gland and Kidney in Lithium Excretion. II. Salivary, Renal and Systemic Clearances of Lithium under Continuous Stimulation of Salivation in Water Loaded Dogs
- Investigation on Interaction of Fractionated ^3H-Heparin with Plasma Proteins by Gel Filtration Chromatography
- INTER-ORGAN RELATION BETWEEN SALIVARY GLAND AND KIDNEY IN LITHIUM EXCRETION : EFFECTS OF CONTINUOUS STIMULATION OF SALIVATION ON LITHIUM CLEARANCES IN DOGS
- ラット小腸におけるニフェジピンの初回通過代謝
- ラット小腸におけるグリセロールの担体輸送機構の解析
- ソフトカプセル用o/w乳剤タイプ新規基剤の開発 II 犬におけるビタミンK_2含有新規ソフトカプセル製剤の経口投与後の吸収特性
- ラットにおける5-フルオロウラシルの初回通過代謝 : 小腸と肝との相対的寄与
- 薬物の唾液中排泄(第48報)イヌにおけるトリメタジオンの唾液中排泄 - 最適唾液採取時間の検討 -
- ラットにおけるリチウムの腸管膜透過機構
- Effect of Glycerol-Related Compounds on Carrier-Mediated Glycerol Uptake in HCT-15 Human Colon Cancer Cell Line
- ラット小腸 in situ 一回灌流法による doxycycline の腸管吸収の解析
- アフリカツメガエル卵母細胞発現系における aquaporin 9 の glycerol 輸送機構の解析
- 薬物腸管吸収に及ぼすプロバイオティクスの影響を探る
- Carrier-Mediated Transport of Glycerol in the Perfused Rat Small Intestine(Biopharmacy)
- Glycerol Uptake in HCT-15 Human Colon Cancer Cell Line by Na^+-Dependent Carrier-Mediated Transport(Biopharmacy)
- Glycerol Absorption by Na^+-Dependent Carrier-Mediated Transport in the Closed Loop of the Rat Small Intestine(Biopharmacy)
- Functional Characterization of the Carrier-Mediated Transport System for Glycerol in Everted Sacs of the Rat Small Intestine(Biopharmacy)
- ラット肝実質細胞でのグリセロール担体輸送の解析
- ラット小腸刷子縁膜小胞におけるリボフラビンの担体輸送
- ラット腸管からのシクロスポリンのバイオアベイラビリティーの投与部位差
- Isolation of Oleayunnanoside from Fraxinus insularis and Revision of Its Structure to Insularoside-6′ -O-β-D-glucoside
- 42 数種のモクセイ科植物のセコイリドイド配糖体の構造と生合成(口頭発表の部)
- 分画ヘパリンの取り込みに関与するスカベンジャー受容体の多様性
- 遊離ラット小腸上皮細胞におけるriboflavinの担体輸送の解析
- ラット小腸におけるnifedipineの初回通過代謝の評価
- ラット小腸でのglycerolの輸送担体に及ぼすアルコール型薬物の影響
- 分画 heparin の体内動態におよぼす修飾ウシ血清アルブミンの影響 : in vivo と in vitro の関係
- 患者におけるアムロジピン錠投与後の体内動態の母集団解析
- 唾液中排泄における変動要因の研究 : TDMと医薬品開発のために
- ラットにおけるリチウム唾液中排泄に関する検討 : 水分負荷及びナトリウム負荷の影響
- SAMBACOLIGNOSIDE, A NEW LIGNAN-SECOIRIDOID GLUCOSIDE FROM JASMINUM SAMBAC(Communications to the Editor)
- Studies on Monoterpene Glucosides and Related Natural Products. XLII. On the Possibility of the Intermediacy of 10-Hydroxyloganin in the Biosynthesis of Secologanin
- Studies on Monoterpene Glucosides and Related Natural Products. XLI. Chemical Conversion of Geniposide into 10-Hydroxyloganin
- Melting Granulation by Addition of Polyethyleneglycol for Stabilization of TAT-59
- Effects of Grinding and Tableting on Physicochemical Stability of an Anticancer Drug, TAT-59
- Anticancer Activity of Free γ-Linolenic Acid on AH-109A Rat Hepatoma Cells and the Effect of Serum Albumin on Anticancer Activity of γ-Linolenic Acid in Vitro
- Hydrolysis of 3', 5'-Dioctanoyl-5-fluoro-2'-deoxyuridine (FdUrd-C8) in Rabbit and Human Plasma and Its Release from Oily Solution
- Cellular Interaction and in Vitro Antitumor Activity of Lipophilic Mitomycin C Prodrugs(Pharmaceutical)
- Decreased Diuretic Response to Furosemide in Rats with Acute Hepatic Failure
- Increased Diuretic Response to Furosemide in Rats with Glycerol-Induced Acute Renal Failure
- URINARY EXCRETION AND DIURETIC RESPONSE TO FUROSEMIDE IN RATS AND HUMANS WITH RENAL INSUFFICIENCY
- Formation of Water-Insoluble Gel in Dry-Coated Tablets for the Controlled Release of Theophylline
- Application of Gel Formation for Taste Masking
- ラットにおけるリチウムの消化管吸収の制御
- Salivary Excretion of 5-Fluorouracil (5-FU). III. : Non-linear Kinetics of Salivary Excretion of 5-FU Following Bolus Intravenous Administration in Rats
- Dose-Dependent Salivary Excretion Following Bolus Intravenous Administration of Lithium in Dog
- Salivary Excretion of 5-Fluorouracil. I. Fluctuation of the Saliva/Plasma Concentration Ratio and Salivary Clearance in Beagle Dogs Following Bolus Intravenous Administration
- Studies on Production of Higher Functional Pharmaceutical Preparations by Using Various Additives. III. Application of Hollow Glass Beads for Intragastric Floating Granules
- Studies on the Development of Intragastric Floating and Sustained Release Preparation. I. Application of Calcium Silicate as a Floating Carrier
- Field Survey of Glycyrrhiza Plants in Central Asia (3) Chemical Characterization of G. glabra Collected in Uzbekistan
- Field Survey of Glycyrrhiza Plants in Central Asia (2). : Characterization of Phenolics and Their Variation in the Leaves of Glycyrrhiza Plants Collected in Kazakhstan
- Field Survey of Glycyrrhiza Plants in Central Asia (1). Characterization of G. uralensis, G. glabra and the Putative Intermediate Collected in Kazakhstan
- Increased Anticoagulant Activity of Recombinant Thrombomodulin Modified with Glycosaminoglycan
- The Tricyclic Antidepressant Clomipramine Increases Plasma Glucose Levels of Mice
- Involvement of Serotonin in Zimelidine-Induced Hyperglycemia in Mice
- Inter-organ Relation between Salivary Gland and Kidney in Lithium Excretion. IV. Saturation of Inhibitory Effect of NaCl on Ductal Reabsorption of Li^+ in Beagle Dogs
- Inter-organ Relation between Salivary Gland and Kidney in Lithium Excretion. III. Enhanced Salivary and Systemic Clearance of Li^+ by Oral Administration of NaCl in Dogs
- Salivary Excretion of 5-Fluorouracil (5-FU). V. Effect of 5-FU Concentration in Perfusate on the Salivary Excretion of 5-FU in Perfused Rat Mandibular Gland
- 22B-15 ラットにおける 5-フルオロウラシルの生物学的利用率の変動性に関する検討
- Application of Carbopol^ to Controlled Release Preparations. II. Controlled Release by Use of Carbopol^ as a Matrix Material, as a Coating Material, and as Both
- Identification and Functional Characterization of Rat Riboflavin Transporter 2
- Fractional Absorption of L-Carnitine after Oral Administration in Rats : Evaluation of Absorption Site and Dose Dependency
- Dose Dependency in the Gastrointestinal Absorption of Cefatrizine : Correlation between in Vivo and in Situ
- In Vitro and ex Vivo Ca-Antagonistic Effect of 2-Methoxyethyl (E)-3-phenyl-2-propen-1-yl (±)-1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl) pyridine-3,5-dicarboxylate (FRC-8653), a New Dihydropyridine Derivative
- Effects of a New Dihydropyridine Derivative, FRC-8653,on Blood Pressure in Conscious Spontaneously Hypertensive Rats
- Emulsion Type New Vehicle for Soft Gelatin Capsule Available for Preclinical and Clinical Trials : Stabilization of New Vehicle Containing Vitamin K_2 with PEG 6000
- Emulsion Type New Vehicle for Soft Gelatin Capsule Available for Preclinical and Clinical Trials : Effects of PEG 6000 and PVP K30 on Physicochemical Stability of New Vehicle
- Emulsion Type New Vehicle Available for Preclinical and Clinical Trials : Stability of Characteristics of Particle Size Distribution of New Vehicle
- Development of Emulsion Type New Vehicle for Soft Gelatin Capsule. I. Selection of Surfactants for Development of New Vehicle and Its Physicochemical Properties
- Metabolic Extraction of Nifedipine during Absorption from the Rat Small Intestine
- Are Novel Scavenger-Like Receptors Involved in the Hepatic Uptake of Heparin ?
- Salivary Excretion of 5-Fluorouracil (5-FU). IV. Dependency of Saliva/Plasma Concentration Ratio and Salivary Clearance on Plasma Concentration of 5-FU during Constant-Rate Intravenous Infusion in Rats
- Inter-organ Relation between Salivary Gland and Kidney in Lithium Excretion. I. Effects of Continuous Stimulation of Salivation on Salivary, Renal and Systemic Clearances of Lithium in Dog
- Salivary Excretion of 5-Fluorouracil. II. Fluctuation of Saliva/Plasma Concentration Ratio and Salivary Clearance during a Constant Rate Intravenous Infusion in Beagle Dogs