Molecular Cloning and Characterization of a Multidrug Efflux Pump, SmfY, from Serratia marcescens(Microbiology)
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概要
- 論文の詳細を見る
We cloned a gene smfY for multidrug efflux pump from chromosomal DNA of Serratia marcescens using drug-hypersensitive Escherichia coli KAM32 as the host, and characterized the pump. E. coli KAM32/pESM42 carrying the smfY showed significantly increased MICs of various drugs including DAPI, norfloxacin, benzalkonium chloride, acriflavine and ethidium bromide, compared with the control. We also detected energy-dependent ethidium and acriflavine efflux due to the SmfY. Sequence analysis revealed that the SmfY was a multidrug efflux pump of the MF (Major Facilitator) superfamily transporters. This is the first report of a multidrug efflux pump belonging to the MF superfamily in S. marcescens.
- 社団法人日本薬学会の論文
- 2007-04-01
著者
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OGAWA Wakano
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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KURODA Teruo
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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TSUCHIYA Tomofusa
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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MIZUSHIMA Tohru
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University
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Tsuchiya T
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Tsuchiya Tomofusa
岡山大学 分子微生物学
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Kuroda T
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Kuroda Teruo
岡山大学 分子微生物学
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Ogawa Wakano
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Ogawa Wakano
岡山大学 分子微生物学
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Ogawa Wakano
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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MINATO Yusuke
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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SHAHCHERAGHI Fereshteh
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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CHEN Jing
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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Mima Takehiko
岡山大学 医歯薬学総合研究科
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TSUTSUMI Tomoaki
National Institute of Health Sciences
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Tsuchiya Tomofusa
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Morita Y
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Kayahara Takashi
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Chen J
Faculty Of Education Fukui University
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Chen J
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Minato Yusuke
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Kuroda T
Faculty Of Pharmaceutical Sciences Fukuoka University
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Mizushima Tohru
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Chen Jing
Department Of Environmental Biology Shandong Agricultural University
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Minato Yusuke
Department Of Biosciences And Informatics Faculty Of Science And Technology Keio University
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Kuroda Teruo
Department Of Microbiology Faculty Of Pharmaceutical Science Okayama University
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Chen Jing
Department Of Biological Sciences And Biotechnology Tsinghua Universty And State Key Laboratory Of B
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Tanabe Tomotaka
College Of Pharmaceutical Sciences Matsuyama University
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Kuroda Teruo
Department of Genome Applied Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Shahcheraghi Fereshteh
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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