Transient DNA Relaxation in Escherichia coli Induced by Nalidixic Acid
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概要
- 論文の詳細を見る
We report here the transient relaxation of plasmid DNA by inhibitors of DNA gyrase in Escherichia coli. Relaxation of plasmid DNA in the presence of nalidixic acid, an inhibitor of the A subunit of DNA gyrase, lasted less than 60 min. The effect of nalidixic acid was not observed in a nalA26 mutant, a strain resistant to nalidixic acid due to a mutation in the gyrA gene. Novobiocin, whose target is the B subunit of DNA gyrase, also caused transient DNA relaxation. Resupercoiling of relaxed DNA in the presence of nalidixic acid was inhibited by pretreatment of the cells with chloramphenicol. As mutations of both the rpoH and recA genes did not affect the resupercoiling reaction, this step seems to require protein synthesis that is independent of both heat shock and SOS respones.
- 公益社団法人日本薬学会の論文
- 1997-05-15
著者
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MIZUSHIMA Tohru
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University
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Mizushima Tohru
Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University
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Miki T
Faculty Of Pharmaceutical Sciences Kyushu University
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OHTSUKA Yoshihisa
Department of Microbiology, Faculty of Pharmaceutical Sciences, Kyushu University
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MIKI Takeyoshi
Department of Microbiology, Faculty of Pharmaceutical Sciences, Kyushu University
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SEKIMIZU Kazuhisa
Department of Microbiology, Faculty of Pharmaceutical Sciences, Kyushu University
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Ohtsuka Yuko
General Laboratory Bml Inc.
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Mizushima Tohru
Faculty Of Pharmaceutial Sciences Kyushu University
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Sekimizu K
Univ. Tokyo Tokyo
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Mizushima T
Faculty Of Pharmaceutial Sciences Kyushu University
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Ohtsuka Y
Department Of Microbiology Faculty Of Pharmaceutical Sciences Kyushu University
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