Functional Cloning and Expression of emeA, and Characterization of EmeA, a Multidrug Efflux Pump from Enterococcus faecalis
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概要
- 論文の詳細を見る
A fragment of chromosomal DNA from Enterococcus faecalis ATCC 29212 was cloned using Escherichia coli KAM32 host cells lacking major multidrug efflux pumps. E. coli KAM32 cells were sensitive to many antimicrobial agents, and the transformed cells harboring a recombinant plasmid became resistant to several structurally unrelated antimicrobial agents such as tetraphenylphosphonium chloride, 4', 6-diamidino-2-phenylindole (DAPI), Hoechst 33342, acriflavine, benzalkonium chloride, norfloxacin and ethidium bromide. This suggests that the cloned DNA fragment carries a gene(s) encoding a multidrug efflux pump. Determination of the nucleotide sequence of the cloned DNA revealed a gene designated as emeA. The transformed E. coli cells showed efflux activity of several antimicrobial agents such as DAPI, Hoechst 33342 and acriflavine. Efflux of DAPI via EmeA was strongly inhibited by reserpine.
- 公益社団法人日本薬学会の論文
- 2003-02-01
著者
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KURODA Teruo
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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TSUCHIYA Tomofusa
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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MIZUSHIMA Tohru
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University
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Tsuchiya T
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Tsuchiya Tomofusa
岡山大学 分子微生物学
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Kuroda T
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Kuroda Teruo
岡山大学 分子微生物学
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CHEN Jing
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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Mima Takehiko
岡山大学 医歯薬学総合研究科
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Huda Md.
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Huda Md.nazmul
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Tsuchiya Tomofusa
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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LEE Eun-Woo
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University
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Chen J
Faculty Of Education Fukui University
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Chen J
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Kuroda T
Faculty Of Pharmaceutical Sciences Fukuoka University
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Mizushima Tohru
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Lee Eun-woo
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Chen Jing
Department Of Environmental Biology Shandong Agricultural University
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Kuroda Teruo
Department Of Microbiology Faculty Of Pharmaceutical Science Okayama University
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Chen Jing
Department Of Biological Sciences And Biotechnology Tsinghua Universty And State Key Laboratory Of B
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Tanabe Tomotaka
College Of Pharmaceutical Sciences Matsuyama University
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Kuroda Teruo
Department of Genome Applied Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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