Alternation of Fatty Acid Composition in A pgsA3 Mutant of Escherichia coli

概要

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We examined the fatty acid composition in an Escherichia coli pgsA3 mutant lacking the potential to synthesize phosphatidylglycerolphosphate, a precursor of phosphatidylglycerol. The contents of C18 : 1cis-9 (oleic acid) and C18 : 1cis-11 (cis-vaccenic acid) in the total phospholipids extracted from the pgsA3 mutant growing at 37℃ were higher and that of C14 : 0 was lower than the wild type cells, resulting in a higher level of unsaturation of fatty acids (ratio of unsaturated fatty acids to saturated ones) in the mutant. The higher level of the unsaturated fatty acids in the pgsA3 mutant was more obvious in cardiolipin than in phosphatidylethanolamine. On the other hand, at 28℃, at which the pgsA3 mutant shows limited cell growth, the content of unsaturated fatty acids in cardiolipin decreased in the pgsA3 mutant compared with the wild type. We consider that the pgsA3 mutant maintains cellular homeostasis by altering the level of unsaturated fatty acids in cardiolipin, and the mechanism is influenced by temperature.
公益社団法人日本薬学会の論文
1997-05-15