Preparation of Specific Antiserum to 17α-Estradiol 17-N-Acetylglucosaminide
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概要
- 論文の詳細を見る
A specific enzyme immunoassay (EIA) method has been developed for 17α-estradiol 17-N-acetylglucosaminide as a model compound instead of 15α-hydroxyestrogen 15-N-acetylglucosaminides. Two new haptens, 3-(ω-carboxyalkyl) ether derivatives of 17α-estradiol 17-N-acetylglucosaminide, were synthesized and conjugated with bovine serum albumin (BSA). the EIa system was newly established using specific antiserum elicited against 3-(1-carboxypropyl) ether of 17α-estradiol 17-N-acetylglucosaminide (17NAG CPE)-BSA conjugate and β-galactosidase-labeled 17NAG CPE as a labeled antigen.An appropriate dose-response curve of EIA for 17α-estradiol 17-N-acetylglucosaminide was obtained in the range of 20-1000 pg/tube. The specificity of EIA proved to be satisfactory in terms of cross-reactivities to related compunds including 15α-N-acetylglucosaminides. The proposed method will be applicable to the preparation of antisera for use in EIA of 15α-hydroxyestrogen 15-N-acetylglucosaminides.
- 公益社団法人日本薬学会の論文
- 1996-11-15
著者
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Matsuki Yasuhiko
Laboratory Of Analytical Chemistry Hatano Research Institute Food And Drug Safety Center
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Matsuki Yasuhiko
Hatano Research Institute Food And Drug Safety Center:pharmaceutical Institute Tohoku University
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Matsuki Yasuhiko
Hatano Research Institute Food And Drug Safety Center
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Suzuki E
Hoechst Marion Roussel Ltd. Kawagoe Jpn
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Nambara Toshio
Hatano Research Institute Food And Drug Safety Center:pharmaceutical Institute Tohoku University
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Nambara Toshio
Hatano Research Institute Food And Drug Safety Center
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Yamawaki Chiyo
Hatano Research Institute Food And Drug Safety Center
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Suzuki Ei-ichiro
Institute Of Life Sciences Ajinomoto Co Inc
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GOTO Junichi
Pharmaceutical Institute, Tohoku University
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SUZUKI Emako
Hatano Research Institute, Food and Drug Safety Center
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NAKAGOMI Madoka
Hatano Research Institute, Food and Drug Safety Center
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TSURUTA Satoko
Hatano Research Institute, Food and Drug Safety Center
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Goto Junichi
Pharmaceutical Institute Tohoku University
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Tsuruta Satoko
Hatano Research Institute Food And Drug Safety Center
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鈴木 恵理香
Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Nagoya City University
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NAKAGOMI Madoka
Laboratory of Analytical Chemistry, Hatano Research Institute, Food and Drug Safety Center
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