Comparative Abilities and Optimal Conditions for β-Glycosidase Enzymes to Hydrolyse the Glucuronide, Glucoside, and N-Acetylglucosaminide Conjugates of Bile Acids
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概要
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Enzymatic hydrolyses were described for three variants of glycosidic conjugated bile acids with one β-glucuronidase (Helix pomatia), three β-glucosidase (almonds, sweet almonds, and Escherichia coli), and four β-N-acetylglucosaminidase (Jack beans, bovine kidney, human placenta, and Diplococcus pneumoniae) preparations. The substrates include the β-glucuronide, β-glucoside, and β-N-acetylglucosaminide conjugates of bile acids related to hyodeoxycholic, murideoxycholic, chenodeoxycholic, and ursodeoxycholic acids possessing a sugar moiety at position C-3,C-6 or C-7. The comparative abilities and optimal conditions for the β-glycosidases to catalyze the hydrolyses of the substrates were clarified by changing pHs and incubation times. Hydrolysis rates of the bile acid glycosides with β-glycosidase treatments were influenced by both the source of the enzyme preparations and the conjugated position of a sugar moiety in the substrates, and the 3-glucoside and 3-N-acetylglucosaminide conjugates were usually hydrolyzed more efficiently than their corresponding 6- and 7-analogs. Escherichia coli and jack bean enzymes were chosen to hydrolyse the glucosidic and N-acetylglucosaminidic conjugated bile acids, respectively.
- 公益社団法人日本薬学会の論文
- 1997-08-15
著者
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Goto J
Tohoku Univ. Sendai Jpn
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Goto J
The Ion Engineering Research Institute Corporation
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Iida Takashi
Dep. Of Chemistry Coll. Of Humanities & Sciences Nihon Univ.
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IIDA Takashi
College of Humanities and Sciences, Nihon University
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Nambara T
Tohoku Univ. Sendai Jpn
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Nambara T
Graduate School Of Pharmaceutical Sciences Tohoku University
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Nambara Toshio
Hoshi University
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MOMOSE Toshiaki
College of Engineering, Nihon University
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MARUYAMA Jun
College of Engineering, Nihon University
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GOTO Junichi
Pharmaceutical Institute, Tohoku University
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Maruyama J
College Of Engineering Nihon University
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Goto Junichi
Pharmaceutical Institute Tohoku University
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Momose Toshiaki
College Of Engineering Nihon University
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