Transport of fluorescent chenodeoxycholic acid via the human organic anion transporters OATP1B1 and OATP1B3.
スポンサーリンク
概要
- 論文の詳細を見る
This study sought to clarify the contributions of organic anion-transporting polypeptide (OATP) 1B1 and 1B3 to the liver uptake of chenodeoxycholic acid (CDCA). We synthesized a fluorescent version of CDCA, chenodeoxychilyl-(Nepsilon-NBD)-lysine (CDCA-NBD), to characterize transporter-mediated uptake. CDCA-NBD is efficiently transported by OATP1B1 and OATP1B3 with high affinities. The Michaelis-Menten constants for CDCA-NBD uptake by OATP1B1 and OATP1B3 were 1.45 +/- 0.39 microM and 0.54 +/- 0.09 microM, respectively. By confocal laser scanning microscopy, CDCA-NBD, which is taken up by OATP1B1 and OATP1B3, was observed to localize to the cytosol. We also examined the transport of newly synthesized fluorescent bile acids. NBD-labeled bile acids, including cholic acid, deoxycholic acid, lithocholic acid, and ursodeoxycholic acid, were all transported by OATP1B1 and OATP1B3. CDCA-NBD exhibited the highest rate of transport of the five NBD-labeled bile acids examined in OATP1B1- and OATP1B3-expressing cells. Our results suggest that OATP1B1 and OATP1B3 play important roles in CDCA uptake into the liver. Fluorescent bile acids are useful tools to characterize the uptake properties of membrane transporters.
- American Society for Biochemistry and Molecular Biologyの論文
著者
-
Mano Nariyasu
東北大学 薬研究
-
Mano N
Tsukuba Research Laboratories Eisai Co. Ltd.
-
HISHINUMA TAKANORI
Division of Pharmacotherapy, Graduate School of Pharmaceutical Sciences, Tohoku University
-
Goto J
Tohoku Univ. Sendai Jpn
-
Goto J
The Ion Engineering Research Institute Corporation
-
Mano N
Dep. Of Pharmaceutical Sciences Tohoku Univ. Hospital
-
Unno Michiaki
Department Of Gastroenterological Surgery Tohoku University Graduate School Of Medicine
-
Unno Michiaki
Division Of Gastroenterological Surgery Department Of Surgery Tohoku University School Of Medicine
-
Yoshida Hiroshi
Division Of Gastroenterological Surgery Department Of Surgery Tohoku University Graduate School Of M
-
Oikawa Masaya
Division Of Gastroenterological Surgery Department Of Surgery Tohoku University Graduate School Of M
-
Abe Tadayoshi
Department Of Gastroenterological Surgery Tohoku University Graduate School Of Medicine
-
Goto J
Division Of Clinical Pharmacy Graduate School Of Pharmaceutical Sciences Tohoku University
-
SAGA Toshihide
Division of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Tohoku University
-
Mano Nariyasu
Analytical Chemistry And Pharmaceutical Research Section Tsukuba Research Laboratories Eisai Co. Ltd
-
Hayashi T
Department Of Pharmacy Fujita Health University Hospital
-
Abe Takaaki
Division Of Nephrology Endocrinology And Vascular Medicine Department Of Medicine Tohoku University
-
Saga Toshihide
Division Of Clinical Pharmacy Graduate School Of Pharmaceutical Sciences Tohoku University
-
Abe Takaaki
Div. Of Nephrology Endocrinology And Vascular Medicine Tohoku Univ. Graduate School Of Biomedical En
-
MIKKAICHI Tsuyoshi
Division of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Tohoku University
-
Aoki Takeshi
Division Of Nephrology Endocrinology And Vascular Medicine Tohoku University Graduate School Of Medi
-
Mikkaichi Tsuyoshi
Jst‐presto Kawaguchi Jpn
-
Mikkaichi Tsuyoshi
Presto Japan Science And Technology Corporation (jst)
-
Hishinuma T
Division Of Clinical Pharmacy Graduate School Of Pharmaceutical Sciences Tohoku University
-
Goto Junichi
Department of Pharmaceutical Sciences, Tohoku University Hospital
-
Okada Masahiro
Division of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Tohoku University
-
Abe Takaaki
Division of Nephrology, Endocrinology and Vascular Medicine, Tohoku University, Graduate School of Medicine
-
Yamaguchi Hiroaki
Department of Pharmaceutical Sciences, Tohoku University Hospital
関連論文
- Immunohistochemistry of a Proliferation Marker Ki67/MIB1 in Adrenocortical Carcinomas : Ki67/MIB1 Labeling Index Is a Predictor for Recurrence of Adrenocortical Carcinomas
- The Nutrient Formula Containing Eicosapentaenoic Acid and Docosahexaenoic Acid Benefits the Fatty Acid Status of Patients Receiving Long-Term Enteral Nutrition
- Design, Production, and Characterization of Recombinant Neocarzinostatin Apoprotein in Escherichia coli
- Phenobarbital Molecularly Imprinted Polymer Selectively Binds Phenobarbital
- Metabolomic profiling of uremic solutes in CKD patients
- Absolute Quantification of Four Isoforms of the Class I Phosphoinositide-3-kinase Catalytic Subunit by Real-Time RT-PCR(Communications to the Editor)
- The Organic Anion Transporter (OATP) Family
- Rapid screening of antineoplastic candidates for the human organic anion transporter OATP1B3 substrates using fluorescent probes
- LC/ESI-tandem mass spectrometric determination of bile acid 3-sulfates in human urine 3β-Sulfooxy-12α-hydroxy-5β-cholanoic acid is an abundant nonamidated sulfate
- クロマトグラフィー科学会学会賞受賞記念論文 Development of Highly Selective Analytical Systems for Biological Substances Using Chromatography Combined with Mass Spectrometry--With Special Reference to Bio-Analytical Studies of Bile Acids
- Synthesis of 3α, 7α, 14α-Trihydroxy-5β-cholan-24-oic Acid : A Potential Primary Bile Acid in Vertebrates
- Potential Bile Acid Metabolites. 25. Synthesis and Chemical Properties of Stereoisomeric 3α, 7α, 16- and 3α, 7α, 15-Trihydroxy-5β-cholan-24-oic Acids
- Human liver-specific organic anion transporter-2 is a potent prognostic factor for human breast carcinoma
- Valproic Acid Elimination Rate and Urinary Excretion of Its Glucuronide Conjugate in Patients with Epilepsy
- Urinary Metabolites of Valproic Acid in Epileptic Patients
- The Role of Chromosome 18 Abnormalities in the Progression of Pancreatic Adenocarcinoma
- Gene therapy for pancreatic cancer based on genetic characterization of the disease
- Fundamental Studies on Acceleration of Charged Micro Particles (Macrons) and Its Interaction with Materials Surfaces by Implantation
- Correct Diagnosis of Vascular Encasement and Longitudinal Extension of Hilar Cholangiocarcinoma by Four-Channel Multidetector-Row Computed Tomography
- Bile acids repress E-cadherin through the induction of Snail and increase cancer invasiveness in human hepatobiliary carcinoma
- Peroxisome proliferator-activated receptor α activates cyclooxygenase-2 gene transcription through bile acid transport in human colorectal cancer cell lines
- Localization of Aldosterone-Producing Adrenocortical Adenomas : Significance of Adrenal Venous Sampling
- Preoperative assessment of hilar cholangiocarcinoma by multidetector row computed tomography
- Expression of MSX2 predicts malignancy of branch duct intraductal papillary mucinous neoplasm of the pancreas
- Successful Treatment of Intraoperative Heart Failure Caused by Ampulla Cardiomyopathy by Intra-aortic Balloon Pumping and Percutaneous Cardiopulmonary Support : Report of a Case
- Simultaneous determination of pancuronium, vecuronium and their related compounds using LC-ESI-MS
- Determination of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) using LC-ESI-MS method and application to hemolyzed blood
- Determination of urinary 12(S)-hydroxyeicosatetraenoic acid by liquid chromatography-tandem mass spectrometry with column-switching technique : sex difference in healthy volunteers and patients with diabetes mellitus
- Inhibitory Action of Oren-gedoku-to Extract on Enzymatic Lipid Peroxidation in Rat Liver Microsomes
- Structural Analysis of N-Linked Carbohydrate Chains of Funnel Web Spider (Agelenopsis aperta) Venom Peptide Isomerase
- Institutional indications for islet transplantation after total pancreatectomy
- Gallbladder agenesis with a stone in the cystic duct bud
- Intraductal papillary-mucinous adenocarcinoma in the remnant pancreas after pancreatoduodenectomy for cancer of Vater's papilla associated with intraductal papillary-mucinous adenoma
- Cysteinyl Leukotrienes Enhance the Degranulation of Bone Marrow-Derived Mast Cells through the Autocrine Mechanism
- Relationship between the Association Constant and Enantioselectivity on the Flavoprotein-Conjugated Chiral Stationary Phase for High-Performance Liquid Chromatography
- Preparation of Neocarzinostatin Apoprotein Mutants and the Randomized Library on the Chromophore-Binding Cavity(Miscellaneous)
- 4 Pro-R Hydrogen of NADPH was Abstracted for Enzymatic Hydride Transfer by N-Ethylmaleimide Reductase of Yarrowia lipolytica
- Angiographic Index for Angioplasty-Treatable Atheromatous Renal Artery Stenosis
- Mesangial proliferative glomerulonephritis with aldosterone-producing adenoma
- Renal Artery Dissection after Angiographic Evaluation of Lower Extremities
- Successful Treatment of Severe Hypertension with the Combination of Angiotensin Converting Enzyme Inhibitor and Angiotensin II Receptor Blocker
- How Much of the Pancreatic Head Should We Resect in Frey's Procedure?
- Adenovirus Expressing Mutant p27^ Enhanced Apoptosis and Inhibited the Growth of Xenografted Human Breast Cancer
- Genotyping of the N-acetyltransferase2 Polymorphism in the Prediction of Adverse Drug Reactions to Isoniazid in Japanese Patients
- Hypocalcemia in a patient with severe hypertension and surgically induced relative hypoparathyroidism
- Advanced bile duct carcinoma in a 15-year-old patient with pancreaticobiliary maljunction and congenital biliary cystic disease
- Determination of Serum Concentrations of Glycyrrhizin in Humans by Semi-micro High-Performance Liquid Chromatography after Administration of a Therapeutic Dose
- Intra- and Inter-Day Variation of LTE4 Levels in Healthy Human Urine Using the Liquid Chromatography/Tandem Mass Spectrometric Method (質量分析総合討論会特集号)
- Determination of leukotriene E_4 in human urine using liquid chromatography-tandem mass spectrometry
- Preparation of a Monoclonal Antibody Specific for 5-Methyl-2'-deoxycytidine and Its Application for the Detection of DNA Methylation Levels in Human Peripheral Blood Cells
- A Sandwich Enzyme-linked Immunosorbent Assay for Δ^3, Δ^2-Enoyl-CoA Isomerase in Rat-Tissue Homogenates
- MOLECULAR CLONING OF THE nemA GENE ENCODING N-ETHYLMALEIMIDE REDUCTASE FROM Escherichia coli
- Simultaneous Detection of Cholyl Adenylate and Coenzyme A Thioester Utilizing Liquid Chromatography/Electrospray Ionization Mass Spectrometry
- Separation and Detection of Bile Acid 24-Glucuronides in Human Urine by Liquid Chromatography Combined with Electrospray Ionization Mass Spectrometry
- Hemodynamic patterns of phosphatidylcholine hydroperoxide and hyaluronic acid during hepatic ischemia-reperfusion
- Direct Injection Method for Quantitation of Endogenous Leukotriene E_4 in Human Urine by Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry with a Column-switching Technique
- Dilation of Renal Artery Stenosis after Administration of Losartan
- Predictors of Undiagnosed Renal Artery Stenosis among Japanese Patients with Risk Factors of Atherosclerosis
- Brain and Heart Specific Alteration of Methamphetamine (MAP) Distribution in MAP-Sensitized Rat
- Farnesoid X receptor, hepatocyte nuclear factors 1α and 3β are essential for transcriptional activation of the liver-specific organic anion transporter-2 gene
- Malignant Pheochromocytoma with Hepatic Metastasis Diagnosed 10 Years After a Resection of the Primary Incidentaloma Adrenal Lesion : Report of a Case
- Screening of Antibiotics That Interact with Organic Anion-Transporting Polypeptides 1B1 and 1B3 Using Fluorescent Probes
- Enzyme Immunoassay for Ursodeoxycholic Acid 7-N-Acetylglucosaminides in Human Urine
- An Improved Method for the Capillary Gas Chromatographic Derivatization of Polyhydroxylated Steroids Having tert-Hydroxyl Groups
- An Experiment for Material Surface Modification by the Charged Microparticle (Macron) Acceleration and Implantation
- Comparative Abilities and Optimal Conditions for β-Glycosidase Enzymes to Hydrolyse the Glucuronide, Glucoside, and N-Acetylglucosaminide Conjugates of Bile Acids
- Major hepatectomy for perihilar cholangiocarcinoma
- Inhibition of Heme Oxygenase Ameliorates Sepsis-Induced Liver Dysfunction in Rats
- Production and Characterization of Monoclonal Antibodies Recognizing the Asymmetric Center of Optically Active Bufuralol and Its Metabolites
- The HMG-CoA Reductase Inhibitor Pravastatin Stimulates Insulin Secretion through Organic Anion Transporter Polypeptides
- Transport of Estrone 3-sulfate Mediated by Organic Anion Transporter OATP4C1 : Estrone 3-sulfate binds to the Different Recognition Site for Digoxin in OATP4C1
- Stage of chronic kidney disease is an outcome-predicting factor of angioplasty for atheromatous renal artery stenosis
- Simplified hepatic resection utilizing absorbable polyglycolic acid-based tape and other ligature apparatus
- Assessment of Frey procedures : Japanese experience
- LC-MS/MS coupled with immunoaffinity extraction for determination of estrone, 17β-estradiol and estrone 3-sulfate in human plasma
- Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry
- Significance of xanthine oxidase in the production of intracellular oxygen radicals in an in-vitro hypoxia-reoxygenation model
- Serous Cystic Neoplasms of the Whole Pancreas in a Patient with von Hippel-Lindau Disease
- 2 Atherosclerotic Renal Arterial Stenosis(Treatment of Atherosclerotic Renal Artery Stenosis-From the View Point of Cardiorenal Protection-, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Calcium-binding protein S100P is a novel diagnostic marker of cholangiocarcinoma
- Evaluation of MSX2 mRNA in brush cytology specimens distinguished pancreatic carcinoma from chronic pancreatitis
- Segmentectomy of the liver
- Paclitaxel as second-line chemotherapy in patients with gemcitabine-refractory pancreatic cancer : a retrospective study
- Metabolomic profiling of the autosomal dominant polycystic kidney disease rat model
- Transport of fluorescent chenodeoxycholic acid via the human organic anion transporters OATP1B1 and OATP1B3.
- Distal pancreatectomy (with video)
- Transport of estrone 3-sulfate mediated by organic anion transporter OATP4C1: estrone 3-sulfate binds to the different recognition site for digoxin in OATP4C1.
- Middle pancreatectomy
- Clinical Characteristics of Gastric Cancer in Patients with Familial Adenomatous Polyposis
- Clinical features and treatment outcome of borderline resectable pancreatic head/body cancer : a multi-institutional survey by the Japanese Society of Pancreatic Surgery