Determination of Serum Concentrations of Glycyrrhizin in Humans by Semi-micro High-Performance Liquid Chromatography after Administration of a Therapeutic Dose
スポンサーリンク
概要
- 論文の詳細を見る
A simple and sensitive semi-micro high-performance liquid chromatography (HPLC) was established for determining the serum levels of glycyrrhizin (GL) in humans. Butyl p-hydroxybenzoate was used as the internal standard and serum was deproteinized by methanol. The samples were separated on Capcell Pak C18 UG120 column (150×1.5 mm i.d.; particle size, 5 μm). The detection limit of GL in serum was 100 ng/ml, which enables determination of serum levels of GL after administration of a therapeutic dose. This time-course study suggested that the elimination rate of GL differed between subjects for the same administered dose, although the sample was too small to allow a meaningful comment. In clinical practice, GL is used for its antiviral and anti-inflammatory effects. Excessive administration of GL can induce pseudoaldosteronism; however the optimal GL concentration in serum remains to be determined. The determination method reported here is expected to aid in the safe and efficient use of the drug in clinical practice.
- 公益社団法人日本薬学会の論文
- 2000-08-01
著者
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Suzuki Naoto
Department of Biochemistry, Nihon University School of Dentistry
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MIZUGAKI Michinao
Department of Clinical Pharmaceutics, Tohoku Pharmaceutical University
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HISHINUMA TAKANORI
Division of Pharmacotherapy, Graduate School of Pharmaceutical Sciences, Tohoku University
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TOMIOKA Yoshihisa
Department of Pharmaceutical Sciences, Tohoku University Hospital and Division of Clinical Pharmacy,
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HISHINUMA Takanori
Department of Pharmaceutical Sciences, Tohoku University Hospital and Division of Clinical Pharmacy,
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ISHIWATA Shunji
Department of Pharmaceutical Sciences, Tohoku University Hospital and Division of Clinical Pharmacy,
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NIIZEKI Masahiro
Department of Pharmaceutical Sciences, Tohoku University Hospital
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Mizugaki Michinao
Department Of Clinical Pharmaceutics Tohoku Pharmaceutical University
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Mizugaki Michinao
Dept. Pharmaceutical Sciences Akita University
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Hayashi T
Department Of Pharmacy Fujita Health University Hospital
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Suzuki N
Laboratory Of Pharmacotherapeutics Department Of Pharmaceutical Sciences Josai International Univers
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NAKASHITA Kei
Department of Pharmaceutical Sciences, Tohoku University Hospital
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KANEKO Shuko
Department of Pharmaceutical Sciences, Tohoku University Hospital
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Nozaki Seishiro
Department Of Pharmaceutical Sciences Tohoku University Hospital And Division Of Clinical Pharmacy G
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Nozaki Seishiro
Department Of Pharmacy Ohta Nishinouchi Hospital
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Ishiwata Shunji
Department Of Pharmaceutical Sciences Tohoku University Hospital And Division Of Clinical Pharmacy G
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Ishiwata Shunji
Faculty Of Pharmaceutical Sciences Kinki University
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Ishiwata Shunji
Department Of Bioanalytical Pharmaceutics Faculty Of Pharmaceutical Sciences Kinki University
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Tomioka Yoshihisa
Division Of Clinical Pharmacy Graduate School Of Pharmaceutical Sciences Tohoku University:laborator
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Tomioka Yoshihisa
Department Of Pharmaceutical Sciences Tohoku University Hospital
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Hishinuma Takanori
Department Of Hygienic Chemistry Pharmaceutical Institute Tohoku University
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Mizugaki M
Department Of Clinical Pharmaceutics Tohoku Pharmaceutical University
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Mizugaki M
Department Of Pharmaceutical Sciences Tohoku University Hospital
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Kaneko S
Department Of Pharmaceutical Sciences Tohoku University Hospital
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Kaneko Shuko
Department Of Pharmaceutical Sciences Tohoku University Hospital
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Nakashita Kei
Department Of Pharmaceutical Sciences Tohoku University Hospital
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Hishinuma T
Division Of Clinical Pharmacy Graduate School Of Pharmaceutical Sciences Tohoku University
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Niizeki Masahiro
Department Of Pharmaceutical Sciences Tohoku University Hospital
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Suzuki Naoto
Department Of Biochemistry Nihon University School Of Dentistry
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Suzuki Naoto
Department of Allergy and Respiratory Medicine, Doai Memorial Hospital
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