IL-1α Stimulates the Formation of Osteoclast-like Cells via RANK-RANKL Signaling System by Osteoblasts and Monocytes(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)

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We examined the effect of the inflammatory mediator interleukin-1α (IL-1α) on the expression of macrophage colony-stimulating factor (M-CSF), osteoprotegerin (OPG), and prostaglandin E_2 (PGE_2) in rat osteoblasts, and the indirect effect of IL-1α on the formation of osteoclast-like cells. The expression of M-CSF and OPG were estimated by determining protein levels using Western blot analysis. PGE_2 expression was determined using ELISA. The formation of osteoclast-like cells was estimated using tartrate-resistant acid phosphatase (TRAP) staining of osteoclast precursors in culture with conditioned medium from IL-1α-treated osteoblasts and the soluble receptor activator of NF-kB ligand (RANKL). M-CSF and PGE_2 expression in osteoblasts increased markedly in cells cultured with IL-1α, whereas OPG expression decreased. The conditioned medium containing M-CSF and PGE_2 produced by IL-1α-treated osteoblasts and soluble RANKL increased the TRAP staining of osteoclast precursors. These results suggest that IL-1α stimulated the formation of osteoclast-like cells via an increase in M-CSF and PGE_2 production, and a decrease in OPG production by osteoblasts.
硬組織再生生物学会の論文