Intra- and Inter-Day Variation of LTE4 Levels in Healthy Human Urine Using the Liquid Chromatography/Tandem Mass Spectrometric Method (質量分析総合討論会特集号)
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概要
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Urinary leukotriene E4 (LTE4) levels in healthy volunteers were determined using liquid chromatography/tandem mass spectrometry (LC/MS-MS). LTE4 and its internal standard were extracted by simple solid-phase extraction and analyzed using LC/MS-MS in the selected reaction monitoring (SRM) mode by monitoring the transitions between m/z 438 and m/z 333 for LTE4 and between m/z 441 and m/z 336 for LTE4-d3. We detected LTE4 (64.7±8.3 pg/mg creatinine, n=13) in healthy human urine. Then, we determined the intra- (within one day) and inter-day (for two weeks) variation of the excretion rates of LTE4 in healthy volunteers (n=5). No significant variations in urinary LTE4 excretion rates were observed in both examinations. In conclusion, LC/MS-MS is a rapid, accurate and sensitive method for determination of urinary LTE4. The present results about urinary LTE4 levels and variation of LTE4 excretion in healthy volunteers can be helpful in evaluating the role of cysteinyl LTs in pathophysiological conditions.
- 日本質量分析学会の論文
- 2000-04-01
著者
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Suzuki Naoto
Department of Biochemistry, Nihon University School of Dentistry
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MIZUGAKI Michinao
Department of Clinical Pharmaceutics, Tohoku Pharmaceutical University
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HISHINUMA Takanori
Department of Pharmaceutical Sciences, Tohoku University Hospital and Division of Clinical Pharmacy,
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Mizugaki Michinao
Department Of Clinical Pharmaceutics Tohoku Pharmaceutical University
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Mizugaki Michinao
Dept. Pharmaceutical Sciences Akita University
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Hayashi T
Department Of Pharmacy Fujita Health University Hospital
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Suzuki N
Laboratory Of Pharmacotherapeutics Department Of Pharmaceutical Sciences Josai International Univers
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Nozaki Seishiro
Department Of Pharmaceutical Sciences Tohoku University Hospital And Division Of Clinical Pharmacy G
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Nozaki Seishiro
Department Of Pharmacy Ohta Nishinouchi Hospital
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Hishinuma Takanori
Department Of Hygienic Chemistry Pharmaceutical Institute Tohoku University
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Mizugaki M
Department Of Clinical Pharmaceutics Tohoku Pharmaceutical University
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Mizugaki M
Department Of Pharmaceutical Sciences Tohoku University Hospital
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Hishinuma T
Division Of Clinical Pharmacy Graduate School Of Pharmaceutical Sciences Tohoku University
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Suzuki Naoto
Department Of Biochemistry Nihon University School Of Dentistry
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