Gingival Fibroblasts Inhibit Mineralized Bone Nodule Formation by Alveolar Bone-derived Cells In Vitro

元データ 1994-03-25 硬組織再生生物学会

概要

The errects of the conditioned culture medium from rabbit gingival fibroblasts (GF) on minera1ized nodule formation by rabbit alveolar bone-derived (AB) cells were investigated using confluent cultured AB cells. The secreted proteins in the conditioned media from both types of cell were determined using sodium dodecyl sulrate (SDS)-polyacrylamide gel electrophorcsis (SDS-PAGE). The cells were cultured in α-minimum essential medium (α-MEM) with and without [14C1proline and l% (vol/vol) serum for 24h. The protein bands in the GF conditioned medium (GFCM) separated by SDS-PAGE showed it contained larger amounts of proteins with molecular masses of over 200 and 50-70kDa, and yielded higher quantities of [14C] pro1ine-1ncorporated proteins with molecular masses of over 200 and 67 kDa than AB cells. Confluent AB cells were cultured in GFCM diluted with an equal volume of α-MEM supplemented with serum and ascorbic acid for 14 days and the mineralized and unmineralized nodules that fornled were observed with the naked eye and counted. The addition of 50% (vol/vol) GFCM to the AB cell cultures reduced the number of minera1ized nodules significantly. The GFCM was divided into 4 fractions (pools 1 to 4), depending on their molecular masses, using gel-filtration column chromatography. Mineralized nodule formation by AB cells was reduced significantly by adding pools 2 and 3, wllich contained proteins with molecular masses of 67-120 and 13-67 kDa respectively, to the culture. Pool 3 was the most effective inhibitor of nodule minera1ization. These results suggest that GF may coordinate osteogenesis of AB cells via the release of some soluble inhibitory factors with a molccular masses of 50-67 kDa.

著者

MUTO Akira Department of Biochemistry and Molecular Biology, Faculty of Agriculture and Life Science, Hirosaki
Suzuki Naoto Department of Biochemistry, Nihon University School of Dentistry
Muto Akira Department Of Biochemistry Nihon University School Of Dentistry
Muto Akira Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Lief Science Hirosaki Univer
Maeno Masao Department of Biochemistry, Nihon University School of Dentistry
Otsuka Kichibee Department of Biochemistry, Nihon University School of Dentistry
Maeno Masao Departments Of Oral Health Sciences Nihon University School Of Dentistry:dsivision Of Functional Mor
Suzuki Naoto Departments Of Biochemistry Nihon University School Of Dentistry:dsivision Of Functional Morphology
OIKAWA Eiji Department of Biochemistry, Nihon University School of Dentistry
Oikawa Eiji Department Of Biochemistry Nihoun University School Of Dentisty
Otsuka Kichibee Departments Of Biochemistry Nihon University School Of Dentistry:dsivision Of Functional Morphology
Otsuka Kichibee Department Of Biochemistry Nihon University School Of Dentistry
Ogawa Hidenori Department of Biochemistry, Nihon University School of Dentistry
Fujikaa Kenji Department of Periodontology,Nihon University School of Dentistry
Ogawa Hidenori Department Of Biochemistry Nihon University School Of Dentistry
Fujikaa Kenji Department Of Periodontology Nihon University School Of Dentistry
Suzuki Naoto Department Of Biochemistry Nihon University School Of Dentistry
Ogawa Hidenori Department of Applied Physics, Graduate School of Engineering, Tohoku University, Aramaki Aoba, Aoba-ku, Sendai 980-8579, Japan
Suzuki Naoto Department of Allergy and Respiratory Medicine, Doai Memorial Hospital

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