Characteristics of Chondroitin Sulfated Proteoglycans in the Matrix Phase of Human Alveolar Bone

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This study demonstrates the types and characteristics of chondroitin sulfated proteoglycans (CSPGs) in the matrix phase of human alveolar bone (HAB) extracted with guanidine-HCI from EDTA-demineralized HAB fragnrents. Different CSPGS including dermatan sulfated PG were analyzed and identified using three monoclonal antibodies in combination with different chondroitinase digestions. The PGS Were partially purified by gel filtration and then anion-exchange column chromatography under denaturing conditions. The CSPGS in each eluted fraction from the columns were detected by ELISA using a monoclonal antibody (MAb) 2B6 in combination with chondroitinase ABC digestion. 2B6-Reactive fractions of the anion-exchange column were concentrated in three fractions. The types of glycosaminoglycan (GAG) chains of CSPGs were determined by Western blotting using antibodies against GAG stubs on a core protein after enzyme digestion. The main CSPGS in the matrix phase of HAB were comprised of dermatan sulfated PG containing a 45 kDa core protein. There were also small amounts of chondroitin non-sulfated PGS which consisted of 45 and 110 kDa core proteins, but no chondroitin 6-sulfated PG was detected.
硬組織再生生物学会の論文