Daily low-intensity pulsed ultrasound stimulates production of bone morphogenetic protein in ROS 17/2.8 cells
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概要
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Although daily low-intensity pulsed ultrasound (LIPUS) can accelerate osteogenic differentiation of the rat clonal cell line ROS 17/2.8, the molecular mechanism that underlies this phenomenon is unclear. The purpose of this study was to determine which molecules exposed to daily LIPUS treatment stimulate osteogenic differentiation. The cells were cultured in the presence and absence (control) of LIPUS stimulation. LIPUS treatments consisted of 1.5-MHz ultrasound administered at an intensity of 30 mW/cm2, 20 min daily for 7 days. The expression of bone morphogenetic proteins (BMPs) and their receptors involved in osteogenesis were measured using real-time PCR and/or Western blot analysis. Phosphorylation of the mothers against decapentaplegic 1 (Smad1) protein was determined by Western blotting. Daily LIPUS treatment significantly increased the expression of BMP-2, -4, and -7 and their receptors, and also phosphorylation of Smad1. Noggin markedly inhibited the daily LIPUS-induced phosphorylation of Smad1. Our findings demonstrate that the osteogenic activity of daily LIPUS may be mediated by BMPs in ROS 17/2.8 cells. (J Oral Sci 51, 29-36, 2009)
著者
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Ito Koichi
Department Of Biomedical Sciences Hirosaki University Graduate School Of Health Sciences
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Kojima Taro
Department Of Geriatric Medicine Graduate School Of Medicine University Of Tokyo
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Takayama Tadahiro
Department of Periodontology, Nihon University School of Dentistry
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Ota Noriko
Department of Periodontology, Nihon University School of Dentistry
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Asano Shoichi
Department of Periodontology, Nihon University School of Dentistry
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Suzuki Naoto
Department of Allergy and Respiratory Medicine, Doai Memorial Hospital
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Suzuki Akito
Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
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