Reconstitution of the Immune System after Murine Allogeneic Umbilical Cord Blood Transplantation
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概要
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The ability of murine allogeneic umbilical cord blood cells （UCBCs） to reconstitute the immune systemwas investigated. UCBCs obtained from fetuses of C57BL/6 （B6; H-2b） mice, which were transgenic for greenfluorescent protein （GFP）, were transplanted into RAG2 （-/-） BALB/c mice （H-2d）. After transplantation, flowcytometric analysis revealed successful reconstitution of phenotypically mature GFP-positive immune cells of donororigin, including T cells, B cells, monocytes, and granulocytes in the peripheral blood of the recipient mice. Analysisof functional maturation of lymphocytes revealed that 2,4,6-trinitrophenyl-keyhole limpet hemocyanin （TNP-KLH）-immunized UCBC-transplanted recipients produced both TNP-specific IgM and IgG antibodies. These resultsindicated that the recipient mice were capable of mounting antibody responses to T-dependent antigens; further, Igclass switching from IgM to IgG confi rmed that both B cells and CD4+ helper T cells derived from allogeneic UCBCswere immunologically competent. Furthermore, mice transplanted with allogeneic UCBCs accepted skin grafts fromboth B6 and BALB/c mice. However, these chimeric mice completely rejected skin grafts from third party C3H/HeJ （H-2k） mice, indicating the presence of functional CD8+ killer T cells as well as CD4+ helper T cells. In termsof potential clinical application, our results indicate that allogeneic UCBC transplantation can enable recovery of thenormal immune system in recipients.
著者
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佐藤 英明
Department Of Animal Science Faculty Of Agriculture Kyoto University
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Ito Koichi
Department Of Biomedical Sciences Hirosaki University Graduate School Of Health Sciences
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Ito Kyoko
Department Of Biomedical Sciences Hirosaki University Graduate School Of Health Sciences
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Sato Hideaki
Department Of Biomedical Sciences Hirosaki University Graduate School Of Health Sciences
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Sato Hideaki
Department Of Applied Physics Faculty Of Engineering Osaka University
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Ito Koichi
Research Center For Frontier Medical Engineering Chiba University
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Sato Masashi
Department Of Medical Technology Hirosaki University School Of Health Sciences
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Sato Masashi
Department Of Applied Biological Science Kagawa University
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Chiba Makie
Department of Medical Technology, Hirosaki University School of Health Sciences
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Chiba Makie
Department Of Medical Technology Hirosaki University School Of Health Sciences
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佐藤 雅
Department of Medical Technology, Hirosaki University School of Health Sciences
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千葉 真希枝
Department of Medical Technology, Hirosaki University School of Health Sciences
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