Preparation of a Monoclonal Antibody Specific for 5-Methyl-2'-deoxycytidine and Its Application for the Detection of DNA Methylation Levels in Human Peripheral Blood Cells

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概要

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• A monoclonal antibody specific for a modified nucleoside, 5-methyl-2'-deoxycytidine (m5dCyd), was prepared using 5-methylcytidine (m5Cyd)-keyhole limpet haemocyanin (KLH) conjugate, and was characterized. Termed FMC9,the antibody reacts with m5dCyd and slightly with m5Cyd and 5-methylcytosine (m5Cyt) but not with other nucleosides tested in this investigation. FMC-9 was used in an enzyme-linked immunosorbent assay (ELISA) system for the quantitation of m5dCyd levels. Sensitivity was in the picomole range. Methylation levels in peripheral blood cells of healthy donors were determined by inhibition ELISA. The percentage of m5dCyd in peripheral blood cells of 10 healthy donors was 5.08±0.50%. These results suggest that the inhibition ELISA using FMC9 is useful to monitor m5dCyd levels in the peripheral blood cells.

• 公益社団法人日本薬学会の論文
• 1996-12-15

著者

• MIZUGAKI Michinao

  Department of Clinical Pharmaceutics, Tohoku Pharmaceutical University

• HISHINUMA TAKANORI

  Division of Pharmacotherapy, Graduate School of Pharmaceutical Sciences, Tohoku University

• ITOH Kunihiko

  Department of Clinical Pharmacology and Genetics, School of Pharmaceutical Science, University of Sh

• HISHINUMA Takanori

  Department of Pharmaceutical Sciences, Tohoku University Hospital and Division of Clinical Pharmacy,

• ISHIWATA Shunji

  Department of Pharmaceutical Sciences, Tohoku University Hospital and Division of Clinical Pharmacy,

• Itoh K

  Medicinal Chemistiy Research Laboratories I Pharmaceutical Research Division Takeda Chemical Industr

• Itoh K

  Pharmaceutical Research Laboratories Iii Pharmaceutical Research Division Takeda Chemical Industries

• ISHIDA NAKAO

  The Sendai Institute of Microbiology

• Mizugaki Michinao

  Department Of Clinical Pharmaceutics Tohoku Pharmaceutical University

• Hayashi T

  Department Of Pharmacy Fujita Health University Hospital

• Yamaguchi T

  Department Of Biological Science Teikyo University Of Science And Technology

• Suzuki N

  Laboratory Of Pharmacotherapeutics Department Of Pharmaceutical Sciences Josai International Univers

• YAMAGUCHI Takahumi

  Department of Pharmaceutical Sciences, Tohoku University Hospital

• NOZAKI Seishiro

  Pharmacy Division, Ohta Nishinouchi Hospital

• Nozaki Seishiro

  Department Of Pharmaceutical Sciences Tohoku University Hospital And Division Of Clinical Pharmacy G

• Nozaki Seishiro

  Department Of Pharmacy Ohta Nishinouchi Hospital

• Itoh K

  Department Of Pharmaceutical Sciences Tohoku University Hospital And Division Of Clinical Pharmacy G

• Itoh Kunio

  Department Of Drug Metabolism And Pharmacokinetics Tohoku Pharmaceutical University

• Ishiwata Shunji

  Department Of Pharmaceutical Sciences Tohoku University Hospital And Division Of Clinical Pharmacy G

• Ishiwata Shunji

  Faculty Of Pharmaceutical Sciences Kinki University

• Ishiwata Shunji

  Department Of Bioanalytical Pharmaceutics Faculty Of Pharmaceutical Sciences Kinki University

• Ishida N

  The Sendai Institute Of Microbiology

• Itoh Kunihiko

  Dept. Pharmaceutical Sciences Akita University

• Hishinuma Takanori

  Department Of Hygienic Chemistry Pharmaceutical Institute Tohoku University

• Mizugaki M

  Department Of Clinical Pharmaceutics Tohoku Pharmaceutical University

• Mizugaki M

  Department Of Pharmaceutical Sciences Tohoku University Hospital

• Itoh Kunihiko

  Department Of Clinical Pharmacology & Genetics School Of Pharmaceutical Sciences University Of S

• Itoh Kunihiko

  Department Clinical Pharmacology & Genetics, School of Pharmaceutical Sciences, University of Shizuoka

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