A Rapid and Simple Detection of Genetic Defects Responsible for the Phenotypic Polymorphism of Cytochrome P450 2C19
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概要
- 論文の詳細を見る
A rapid and simple cytochrome P450 (CYP) 2C19 genotyping system was established by making several modifications in previously reported procedures. PCR conditions were modified to be capable of simultaneous amplification of CYP2C19ml and CYP2C19m2 regions. Intensive bands of 169 bp for the CYP2C19ml region and 329 bp for the CYP2C19m2 region with low background were obtained by using PCR condition involving an initial denaturation of 5 min at 94℃, 35 cycles of 1 min at 94℃; 1 min at 53℃; 1 min at 72℃, and final extension of 5 min at 72℃. Next, the optimal restriction enzyme digestion conditions were determined by using PCR products from a subject of wt/wt genotype. Both products were completely digested with 5 U of the corresponding enzyme (Sma I for CYP2C19ml and Bam HI for CYP2C19m2) for 1 h incubation at an optimal temperature. The incidence (16%; 5/32) of subjects homozygous for mutant alleles determined by an established assay system agreed well with the incidence of the poor metabolizer (PM) phenotype in the Japanese population. The established genotyping system would, therefore, be applicable to the clinical laboratory testing of patients with a PM phenotype of CYP2C19 to select appropriate and effective medication.
- 公益社団法人日本薬学会の論文
- 1999-01-15
著者
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SUZUKI Toshio
Department of Animal Science, Faculty of Agriculture, Utsunomiya University
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ITOH Kunihiko
Department of Clinical Pharmacology and Genetics, School of Pharmaceutical Science, University of Sh
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INOUE Kazuyuki
Department of Clinical Pharmacology and Genetics, School of Pharmaceutical Sciences, University of S
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SHISHIDO Takao
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University
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Suzuki Takehiro
Department Of Pharmaceutical Science Akita University Hospital
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Suzuki Toshio
Department Of Pharmaceutical Science Akita University Hospital
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Itoh Kunihiko
Department Of Clinical Pharmacology And Genetics School Of Pharmaceutical Sciences University Of Shi
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Inoue Kazuyuki
Department Of Clinical Pharmacology And Genetics School Of Pharmaceutical Sciences University Of Shi
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YANAGIWARA Seiko
Akita Red-Cross Hospital
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KYOYA Hiroyuki
Department of Pharmaceutical Science, Akita University Hospital
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Hirooka K
Tohoku Univ. Sendai Jpn
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Itoh Kunihiko
Department Of Clinical Pharmacology & Genetics School Of Pharmaceutical Sciences University Of S
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Shishido T
Department Of Pharmaceutical Science Akita University Hospital
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Suzuki Toshio
Department Of Agronomy Faculty Of Agriculture Tohoku University
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Suzuki Toshio
Department Of Metallurgy And Materials Science School Of Engineering The University Of Tokyo
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Inoue Kazuyuki
Department Of Clinical Pharmacology & Genetics School Of Pharmaceutical Sciences University Of S
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Suzuki Takehiro
Department Of Urology Akita University School Of Medicine
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Itoh Kunihiko
Department Clinical Pharmacology & Genetics, School of Pharmaceutical Sciences, University of Shizuoka
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Inoue Kazuyuki
Department Clinical Pharmacology & Genetics, School of Pharmaceutical Sciences, University of Shizuoka
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