IMMUNOHISTOCHEMICAL INVESTIGATION OF AMINOPEPTIDASE
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概要
- 論文の詳細を見る
The localization of aminopeptidase was examined on the porcine intestine by use of fluorescent antibody method. Aminopeptidase was present diffusely in the striated border of mucous epithelial cell of jejunum and ileum, supra-nuclear region of epithelial cell, intestinal glandular cells and in goblet cell. The condensed materials in the crypts showed specific fluorescence. The antigen was not always observed in every cell mentioned above, and the distribution pattern of antigen varied according to the stage of digestion. The enzyme was concentrated in the goblet cells of the small intestine but not in those of the large intestine. Brunner's gland of duodenum did not contain any aminopeptidase. Though aminopeptidase in kidney and pancreas had immunological common factor with that in the intestine, specific fluorescence was not observed on the tissue sections. For comparative purposes, histochemical staining was carried out by means of Burstone and Folk's method. As a result, the goblet cell of the small intestine did not show any cytochemical reaction, though the specific fluorescence which showed the site of the enzyme protein was concentrated in it. As far as the striated border of both mucous epithelial cell and intestinal glandular cell is concerned, the results of the cytochemical reaction coincided with those obtained by immunohistochemical study. The reason for the discordance with regard to the distribution of cytochemical reaction and the immunofluorescence in the goblet cell remained unknown.
- 日本組織細胞化学会の論文
著者
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Takano Kunio
Department Of Anatomy School Of Medicine Keio University
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Yasuda Kenjiro
Department Of Anatomy Keio University
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Suzuki Toshio
Department Of Anatomy School Of Medicine Keio University
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Suzuki Toshio
Department Of Agronomy Faculty Of Agriculture Tohoku University
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