PREPARATION OF MONOCLONAL ANTIBODY TO RABBIT KIDNEY GAMMA-GLUTAMYL TRANSPEPTIDASE AND ITS CROSS-REACTIVITY ANALYSIS IN IMMUNOHISTOCHEMISTRY
スポンサーリンク
概要
- 論文の詳細を見る
A monoclonal antibody against rabbit kidney, γ-GTP, was prepared. The subclass of the antibody was IgG_<2a> and the light chain was λ. The anti-body specifically recognized the H chain, the site involved in anchoring part of this enzyme. The immunohistochemical assay detected reaction in the brush border of the proximal convoluted tubule of the rabbit kidney, particularly on the distal part. Cross-reaction between homologous and heterologous organs was imnunohistochemically examined using this antibody. In the homologous organs, reaction was absorbed in the intercalated duct of the rabbit pancreas and along the stereocilia of the ductus epididymidis. No cross-reaction was detected in the kidney, pancreas or epididymis of a rat, or in the human kidney.
- 日本組織細胞化学会の論文
著者
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Yamashita Shuji
Department Of Cardiology Gifu University Graduate School Of Medicine
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YASUDA KENJIRO
Department of Gastroenterology, Kyoto Second Red Cross Hospital
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Yamashita Shinsuke
Department Of Chemistry Naruto University Of Teacher Education
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Yamashina Shohei
Dept. Pharmaceutical Science Tohoku University
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Yasuda K
Laboratory Of Metabolism Graduate School Of Human And Environmental Studies Kyoto University
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Yasuda Kenjiro
Department Of Anatomy Keio University
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Yamashita S
Keio Univ.
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KOMATSU TOSHIKO
Department of Anatomy, School of Medicine, Keio University
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Komatsu Toshiko
Department Of Anatomy School Of Medicine Keio University
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Yamashita Shuji
Department Of Anatomy Kitasato University School Of Medicine
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