Protein Gene Product 9.5 is an useful marker for differentiation of pancreatic endocrine cells
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概要
- 論文の詳細を見る
Anti-PGP9.5 antibody labels endocrine cells of pancreas. In order to elucidate a developmental dynamics of pancreatic endocrine cells, here we studied the distributions of PGP9.5-posotive-cell and pancreatic-hormone producing cell in the developing rat pancreas. Sections of pancreas of Wistar rats from embryonic day-10 (B10) to 6-week after birth were stained with anti-PGP9.5 antibody (Ultraclone) or antibodies against various pancreatic hormones. Some sections were double immunostained for PGP9.5 and hormones. The PGP9.5-positive cells started to appear in Ell pancreatic bud. Most but not all of the PGP9.5-positive cells showed immunoreactivity for glucagons. There were a few insulin-producing cells but neither somatostatin nor pancreatic polypeptide producing cells were seen. Endocrine clusters, ducts, and exocrine acini were developed in E15. All cells in the endocrine portions and a part of duct cells were positive for PGP9.5. The exocrine acinar cells were negative for PGP9.5 during the pancreatic development. In E17, all duct epithelial cells became positive for PGP9.5. Some of them also showed immunoreactivity for insulin. In E19, small but definite islets were established, in which there were glucagon-, insulin-, somatostatin-, and pancreatic polypeptide-positive cells were developed. All their cells were also labeled with anti-PGP9.5 antibody. The PGP9.5-positive cells in duct decreased dramatically in postnatal stage and disappeared by 18-days after birth. In adult, all endocrine cells in the mature islet were stained for PGP9.5. In addition, we found sporadic PGP9.5-positive cells in the excretory duct. From these evidences, PGP9.5 was concluded to be expressed in the progenitor and mature endocrine cells, and was a good differentiation marker of endocrine cells.
- 日本組織細胞化学会の論文
著者
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FUJITA Yuko
Department of Internal Medicine, Divisions of Neurology, Nephrology, and Rheumatology, Nippon Medica
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YAMASHINA Shohei
Department of Anatomy, Kitasato University School of Medicine
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Hayashi Keiko
Department of Medical Technology Nagoya University School of Health Sciences
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KADOYA Yuichi
Department of Anatomy, Kitasato University School of Medicine
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TAMAKI Hideaki
Department of Anatomy, Kitasato University School of Medicine
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Tamaki H
Kitasato Univ. School Of Medicine
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Tamaki Hideaki
Department Of Anatomy Kitasato University School Of Medicine
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Fujita Yuko
Department Of Anatomy Kitasato University School Of Medicine
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Yamashina Shohei
Departments Of Anatomy Kitasato University School Of Medicine
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Yamashina Shohei
Dept. Pharmaceutical Science Tohoku University
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Yamashina Shohei
Department Of Anatomy And Cell Biology Kitasato University School Of Medicine
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Kadoya Yuichi
Department Of Anatomy Kitasato University School Of Allied Health Sciences
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Hayashi Keiko
Department Of Surgery Kitasato University School Of Medicine
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Hayashi Keiko
Department Of Dermatology Faculty Of Medicine University Of Tokyo
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Yamashina Shohei
Departmant Of Anatomy And Cell Biology Tokyo Medical And Dental University
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Hayashi Keiko
Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima
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