INTRACELLULAR LOCALIZATION OF ACID PHOSPHATASE AND TRYPSINOGEN ON AN IDENTICAL SPECIMEN IN RAT PANCREAS

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Fine intracellular localization of acid phosphatase (AcPase) and trypsinogen was investigated in an identical specimen of rat exocrine pancreas. The tissue was embedded in acrylamide gel after AcPase enzyme histochemical reaction, and ultrathin sections were prepared successively from the gel block. The trypsinogen was detected on the cut face of each section by use of the immuno-ferritin method. AcPase activity was recognized in lysosomes, Golgi vacuoles, Golgi vesicles in trans face and in the innermost Golgi lamellae. Trypsinogen occurred in secretory granules and Golgi vacuoles. The Golgi vacuoles were classified into three types on the basis of their contents : i.e., vacuoles containing both AcPase activity and trypsinogen-immunoreactivity, vacuoles containing only trypsinogen-immunoreactivity, and vacuoles showing only AcPase activity. The first type of the Golgi vacuoles was most frequently observed and the third type of Golgi vacuoles, rarely. On the basis of the result, it is concluded that lysosomes and secretory granules develop simultaneously from the trans face of the Golgi apparatus. It is suggested that one role of lysosomal enzymes may be intracellular modification of secretory proteins in the Golgi mature face of rat exocrine pancreas.
日本組織細胞化学会の論文